小鼠乳酸脱氢酶-A同工酶cDNA和假基因的分离与鉴定

Isolation and characterization of a cDNA and a pseudogene for mouse lactate dehydrogenase-A isozyme.

作者信息

Akai K, Yagi K, Tiano H F, Pan Y C, Shimizu M, Fong K, Jungmann R A, Li S S

出版信息

Int J Biochem. 1985;17(5):645-8. doi: 10.1016/0020-711x(85)90298-8.

Abstract

A mouse lactate dehydrogenase-A cDNA was isolated and it was shown to contain the 393bp of the protein-coding sequence and 488bp of the 3' untranslated region. The amino acid sequence deduced from its open reading frame provided independent evidence for the sequence of residues 201-331 of mouse LDH-A subunit (muscle). This cDNA clone was used as a probe to isolate a mouse genomic clone containing a truncated, processed LDH-A pseudogene. This pseudogene showed 81.6% homology at 713 positions compared with the LDH-A cDNA sequence. The divergence of this pseudogene was estimated to have occurred 39 million years ago.

摘要

分离出小鼠乳酸脱氢酶 -A cDNA，结果表明它包含393bp的蛋白质编码序列和488bp的3'非翻译区。从其开放阅读框推导的氨基酸序列为小鼠LDH -A亚基（肌肉）201 - 331位残基的序列提供了独立证据。该cDNA克隆用作探针分离出一个包含截短的、加工过的LDH -A假基因的小鼠基因组克隆。与LDH -A cDNA序列相比，该假基因在713个位置显示出81.6%的同源性。估计该假基因的分化发生在3900万年前。