一个减数分裂后表达的克隆编码乳酸脱氢酶同工酶X。

A postmeiotically expressed clone encodes lactate dehydrogenase isozyme X.

作者信息

Tanaka S, Fujimoto H

出版信息

Biochem Biophys Res Commun. 1986 Apr 29;136(2):760-6. doi: 10.1016/0006-291x(86)90504-8.

Abstract

We have analysed by the DNA sequencing one of the cDNA clones, pPM459, to mRNA abundant in spermatids of mice. This clone contained 535 base pair nucleotides with a coding region for 139 amino acids and a 3' untranslated region including a single polyadenylation signal. Screening of the protein database revealed that the deduced amino acid sequence highly matched the carboxyl terminal residues 192-330 of mouse lactate dehydrogenase isozyme X (LDH-X). Taken together with our previous report which showed transcription of the message hybridizing to pPM459 after meiosis, it was demonstrated that LDH-X mRNA synthesis continued during the postmeiotic phase in spermatogenesis.

摘要

我们通过DNA测序分析了其中一个cDNA克隆pPM459，它对应于小鼠精子细胞中丰富的mRNA。该克隆包含535个碱基对的核苷酸，有一个编码139个氨基酸的编码区和一个3'非翻译区，其中包括一个单一的聚腺苷酸化信号。对蛋白质数据库的筛选显示，推导的氨基酸序列与小鼠乳酸脱氢酶同工酶X（LDH-X）的羧基末端残基192 - 330高度匹配。结合我们之前的报告，即减数分裂后与pPM459杂交的信息发生转录，结果表明在精子发生的减数分裂后阶段，LDH-X mRNA的合成仍在继续。