Fukasawa K M, Tanimura M, Sakai I, Sharief F S, Chung F Z, Li S S
Genetics. 1987 Jan;115(1):177-84. doi: 10.1093/genetics/115.1.177.
The presence of at least ten mouse LDH-A pseudogenes was demonstrated in the genomic blot analysis, and four different processed pseudogenes have thus far been isolated and characterized. In this report, the nucleotide sequences to two different mouse lactate dehydrogenase-A processed pseudogenes, M11 and M14, were determined and compared with the protein-coding sequences of the mouse and rat LDH-A functional genes. In the pseudogene M11, the sequence of 64 nucleotides from codon no. 257 to 278 was tandemly duplicated. In the pseudogene M14, the sequence of 22 nucleotides from codon no. 68 to 75 was replaced by an inserted repetitive sequence of 242 nucleotides homologous to a mouse truncated R element. The pattern of nucleotide substitutions accumulated in mouse LDH-A pseudogenes M11 and M14, as well as that of pseudogene M10 identified previously, was analyzed, and the substitution frequencies of the C or G at the CG dinucleotide were found to be high.
基因组印迹分析证明至少存在十个小鼠乳酸脱氢酶A假基因，目前已分离并鉴定出四种不同的加工假基因。在本报告中，测定了两种不同的小鼠乳酸脱氢酶A加工假基因M11和M14的核苷酸序列，并与小鼠和大鼠乳酸脱氢酶A功能基因的蛋白质编码序列进行了比较。在假基因M11中，从第257号密码子到278号密码子的64个核苷酸序列串联重复。在假基因M14中，从第68号密码子到75号密码子的22个核苷酸序列被一个与小鼠截短的R元件同源的242个核苷酸的插入重复序列所取代。分析了在小鼠乳酸脱氢酶A假基因M11和M14以及先前鉴定的假基因M10中积累的核苷酸取代模式，发现CG二核苷酸处的C或G取代频率很高。
