Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA.
Lab Chip. 2018 Jul 10;18(14):2124-2133. doi: 10.1039/c8lc00196k.
We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density. The fluidic trapping step is achieved by balancing the hydrodynamic resistances of three parallel channel segments forming a microfluidic trifurcation, the purpose of which was to capture single cells in a high-density array. Next, the cells were transferred into adjacent larger compartments by generating an array of streaming micro-vortices to move the cells to the desired streamlines in a massively parallel format. This approach can compartmentalize single cells with efficiencies of ≈67% in compartments that have diameters on the order of ∼100 um, which is an appropriate size for single cell proliferation studies and other single cell biochemical measurements.
我们展示了一种混合微流控系统，该系统结合了流体捕获和声波切换功能，可在高密度下对单细胞进行排列。通过平衡三个平行通道段的流体阻力来实现流体捕获步骤，这三个平行通道段形成了微流控三分叉，其目的是在高密度阵列中捕获单个细胞。接下来，通过生成一系列流动微涡旋将细胞转移到相邻的较大隔室中，以将细胞以大规模平行的方式移至所需的流线中。这种方法可以将单个细胞以约 67%的效率分箱到直径约为 100 µm 的隔室中，这对于单细胞增殖研究和其他单细胞生化测量是合适的尺寸。
