用于青枯雷尔氏菌转座子诱变的自杀载体。
Suicide vector for transposon mutagenesis in Pseudomonas solanacearum.
作者信息
Morales V M, Sequeira L
出版信息
J Bacteriol. 1985 Sep;163(3):1263-4. doi: 10.1128/jb.163.3.1263-1264.1985.
Abstract
A suicide vector was constructed by cloning the transfer genes of the wide-host-range (IncW group) plasmid R388 into the BamHI site of pBR325. This plasmid can deliver Tn5 into Pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.
摘要
通过将广宿主范围（IncW 组）质粒 R388 的转移基因克隆到 pBR325 的 BamHI 位点，构建了一种自杀载体。该质粒能够以每受体 10^(-6) 至 10^(-9) 的频率将 Tn5 导入青枯雷尔氏菌。
Zotero 插件