Massey Institute of Food Science and Technology, Massey University, Riddet Road, 4442 Palmerston North, New Zealand.
School of Engineering & Advanced Technology, Massey University, Riddet Road, 4442 Palmerston North, New Zealand.
J Glob Antimicrob Resist. 2018 Dec;15:25-31. doi: 10.1016/j.jgar.2018.06.003. Epub 2018 Jun 19.
The aim of this study was to determine gene expression associated with the persistence of a Listeria monocytogenes stationary-phase population when facing lethal nisin treatment.
RNA-Seq analysis was used for gene expression profiling of persister cells in nutrient-rich medium (persister TN) compared with untreated cells (non-persister). The results were confirmed using reverse transcription quantitative PCR (RT-qPCR).
Functional genes associated with the persister population were identified in multiple systems, such as heat-shock-related stress response, cell wall synthesis, ATP-binding cassette (ABC) transport system, phosphotransferase system (PTS) and SOS/DNA repair.
This study pointed to genetic regulation of persister cells exposed to lethal nisin concentrations and provides some insight into possible mechanisms of impeding bacterial persistence.
本研究旨在确定李斯特菌（Listeria monocytogenes）在面对致死性乳链菌肽（nisin）处理时，处于静止期种群持续存在相关的基因表达。
采用 RNA-Seq 分析比较富含营养的培养基中（持久 TN）与未处理细胞（非持久）的持久细胞基因表达谱。使用反转录定量 PCR（RT-qPCR）对结果进行了验证。
在多个系统中鉴定出与持久种群相关的功能基因，如热休克相关应激反应、细胞壁合成、ATP 结合盒（ABC）转运系统、磷酸转移酶系统（PTS）和 SOS/DNA 修复。
本研究指出了在致死性乳链菌肽浓度下暴露的持久细胞的遗传调控，并为阻止细菌持久存在的可能机制提供了一些见解。
