Division of Pediatric Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States of Americafs.
Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, California, United States of America.
PLoS One. 2018 Jun 22;13(6):e0196710. doi: 10.1371/journal.pone.0196710. eCollection 2018.
The use of lactobacilli in prevention of necrotizing enterocolitis (NEC) is hampered by insufficient knowledge about optimal species/strains and effects on intestinal bacterial populations. We therefore sought to identify lactobacilli naturally occurring in postnatal rats and examine their ability to colonize the neonatal intestine and protect from NEC. L. murinus, L. acidophilus, and L. johnsonii were found in 42, 20, and 1 out of 51 4-day old rats, respectively. Higher proportion of L. murinus in microbiota correlated with lower NEC scores. Inoculation with each of the three species during first feeding significantly augmented intestinal populations of lactobacilli four days later, indicating successful colonization. L. murinus, but not L. acidophilus or L. johnsonii, significantly protected against NEC. Thus, lactobacilli protect rats from NEC in a species- or strain-specific manner. Our results may help rationalizing probiotic therapy in NEC.
乳酸杆菌在预防坏死性小肠结肠炎（NEC）中的应用受到对最佳物种/菌株和对肠道细菌种群影响的了解不足的阻碍。因此，我们试图确定出生后大鼠体内自然存在的乳酸杆菌，并研究它们定植新生儿肠道和预防 NEC 的能力。在 51 只 4 天大的大鼠中，分别发现了 42 只、20 只和 1 只乳酸乳球菌、嗜酸乳杆菌和约翰逊乳杆菌。肠道微生物群中乳酸乳球菌的比例越高，NEC 评分越低。在首次喂养期间接种这三种菌均可显著增加四天后肠道乳酸杆菌的数量，表明定植成功。乳酸乳球菌，但不是嗜酸乳杆菌或约翰逊乳杆菌，能显著预防 NEC。因此，乳酸杆菌以物种或菌株特异性方式保护大鼠免受 NEC 影响。我们的研究结果可能有助于为 NEC 的益生菌治疗提供依据。
