Oral Babaoğlu Aylin, Soyder Aykut, Malatyalı Erdoğan, Ertuğ Sema, Ertabaklar Hatice
Ege University Faculty of Medicine, Department of Parasitology, Izmir, Turkey.
Adnan Menderes University Faculty of Medicine, Department of General Surgery, Aydin, Turkey.
Mikrobiyol Bul. 2018 Apr;52(2):198-205. doi: 10.5578/mb.66711.
Cyst hydatid (CH) is a zoonotic infection that is characterized by the development of metacestode form of Echinococcus granulosus primarily in liver of humans and ruminants. With a worldwide distribution, the infection is still considered as an important parasitic disease that threatens the public health in Turkey as in the other developing countries. Morphological and biological features of parasite fail to discriminate isolates for typing so molecular methods should be used for this purpose. Recently, a total of eleven genotypes of E.granulosus (G1-10 and lion strain) have been identified and these genotypes are highly correlated with host specificity of the parasite. The aim of this study, was to determine the genotypes of E.granulosus isolates from human samples in Aydın. Cyst fluids from CH operated cases in Adnan Menderes University Faculty of Medicine, Training and Research Hospital were used in the present study. Samples were washed with phosphate buffered saline (PBS) and stored in 70% ethanol at -20ºC. Mitochondrial cytochrome C oxidase subunit 1 (cox1) gene was amplified partially by polymerase chain reaction (PCR). The PCR products were sequenced initially, compared to other database in Genbank and evolutionary distances were estimated with references. The genotypes of E.granulosus isolates were determined according to the closest or exact matches to the references. A total of 20 E.granulosus isolates were genotyped in the present study, most of them (15 isolates, 75%) were identified as Genotype 1 (G1), that is defined as sheep genotype and the remaining isolates were defined as pig/camel genotype G6/7 (five isolates, 25%). A possible explanation to our results may be related to the geographical position of Turkey. The identification of G6/7 in addition to sheep genotype G1 indicated that pigs and camels in this area have role in the transmission and distribution of E.granulosus to humans. There is still limited information about the molecular epidemiology of E.granulosus in Turkey. This study reveals the first data about the genotype distribution of E.granulosus in our city, therefore the findings may help to design control program for the disease with a combination of epidemiological data.
包虫囊肿（CH）是一种人畜共患感染病，其特征是细粒棘球绦虫的中绦期主要在人类和反刍动物的肝脏中发育。这种感染病在全球范围内均有分布，在土耳其以及其他发展中国家，它仍被视为一种威胁公众健康的重要寄生虫病。寄生虫的形态学和生物学特征无法区分用于分型的分离株，因此应使用分子方法来实现这一目的。最近，总共鉴定出了细粒棘球绦虫的11种基因型（G1 - 10和狮株），这些基因型与该寄生虫的宿主特异性高度相关。本研究的目的是确定来自艾登省人类样本的细粒棘球绦虫分离株的基因型。本研究使用了阿德南·曼德列斯大学医学院培训与研究医院接受CH手术病例的囊液。样本用磷酸盐缓冲盐水（PBS）洗涤后，保存在-20℃的70%乙醇中。通过聚合酶链反应（PCR）部分扩增线粒体细胞色素C氧化酶亚基1（cox1）基因。首先对PCR产物进行测序，与Genbank中的其他数据库进行比较，并参考相关数据估计进化距离。根据与参考序列的最接近或完全匹配来确定细粒棘球绦虫分离株的基因型。在本研究中，总共对20株细粒棘球绦虫分离株进行了基因分型，其中大多数（15株，75%）被鉴定为基因型1（G1），即绵羊基因型，其余分离株被定义为猪/骆驼基因型G6/7（5株，25%）。对我们研究结果的一种可能解释可能与土耳其的地理位置有关。除了绵羊基因型G1外还鉴定出G6/7，这表明该地区的猪和骆驼在细粒棘球绦虫向人类的传播和扩散中起到了作用。关于土耳其细粒棘球绦虫分子流行病学的信息仍然有限。本研究揭示了我市细粒棘球绦虫基因型分布的首批数据，因此这些发现可能有助于结合流行病学数据设计该疾病的控制方案。
