Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Bee Industry Technology Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101,China.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; Environment and Plant Protection College, Hainan University, Haikou 570228, China.
Pestic Biochem Physiol. 2018 May;147:133-138. doi: 10.1016/j.pestbp.2017.11.001. Epub 2017 Nov 8.
Myclobutanil is currently used on the flowering plants. Little is known about how Apis cerana cerana respond to myclobutanil exposure. Hence, the acute toxicity of myclobutanil and its sublethal effects on respiration, flight and detoxification enzymes [7-ethoxycoumarin O-deethylase (ECOD) and glutathione S-transferases (GSTs)] in A. cerana cerana were investigated. The results indicated that formulation grade myclobutanil showed moderate toxicity to A. cerana cerana either contact (LD=4.697μg/bee) or oral (LD=2.154μg/bee) exposure. Sublethal dose of myclobutanil significantly reduced the respiration rate of workers at 24h and 48h regardless of the exposure method. However, myclobutanil didn't significantly affect the take-off flight. After nurse bees exposure to the dose (LD) of formulation-grade myclobutanil, ECOD activity was significantly induced when compared with control, but GST activity didn't change. In the forager bees, no enzyme markers response was obtained in this test. From the present study we can infer that myclobutanil disturb respiration and P450-mediated detoxification of the individual bees of A. cerana cerana. Thus, myclobutanil may has risk for A. cerana cerana, it should be cautiously used.
环戊菌唑目前被用于开花植物。然而，人们对中华蜜蜂对环戊菌唑暴露的反应知之甚少。因此，本研究调查了环戊菌唑对中华蜜蜂的急性毒性及其对呼吸、飞行和解毒酶[7-乙氧基香豆素 O-脱乙基酶（ECOD）和谷胱甘肽 S-转移酶（GSTs）]的亚致死效应。结果表明，制剂级环戊菌唑对中华蜜蜂无论是接触（LD=4.697μg/只）还是口服（LD=2.154μg/只）暴露，均表现出中等毒性。亚致死剂量的环戊菌唑无论暴露方式如何，均显著降低工蜂在 24h 和 48h 的呼吸速率。然而，环戊菌唑对起飞飞行没有显著影响。与对照相比，在接触制剂级环戊菌唑剂量后，蜜蜂的 ECOD 活性显著增加，但 GST 活性没有变化。在采集蜂中，在该试验中未获得任何酶标记物的反应。从本研究可以推断，环戊菌唑干扰了中华蜜蜂个体的呼吸和 P450 介导的解毒作用。因此，环戊菌唑可能对中华蜜蜂有风险，应谨慎使用。
