College of Environment and Plant Protection, Hainan University, Haikou 570228, China.
College of Environment and Plant Protection, Hainan University, Haikou 570228, China.
Pestic Biochem Physiol. 2018 May;147:40-45. doi: 10.1016/j.pestbp.2017.08.015. Epub 2017 Aug 31.
Bacillus is the most widely studied biocontrol agent and has been extensively used in the development of biopesticides and fungicides. In this study, a new cyclic lipopeptide was isolated from Bacillus amyloliquefaciens HAB-2 by column chromatography on silica gel and Sephadex LH-20, and its structures was elucidated on the basis of spectroscopic analysis. This compound is a bacillomycin d-like compound, named as bacillomycin DC. The activity of bacillomycin DC was evaluated against C. gloeosporioides Penz. The median inhibitory concentration of bacillomycin DC was 1.21μg/mL. In addition, bacillomycin DC may have low toxicity to aquatic species as indicated by its 96h half maximum lethal concentration of 22.20μg/mL to zebrafish (Danio rerio). Our current study further provides evidence that bacillomycin DC is a potent fungicide against C. gloeosporioides Penz.
芽孢杆菌是研究最多的生物防治剂，已广泛应用于生物农药和杀菌剂的开发。在这项研究中，通过硅胶和 Sephadex LH-20 柱层析从解淀粉芽孢杆菌 HAB-2 中分离到一种新的环状脂肽，并根据光谱分析确定其结构。该化合物是一种芽孢杆菌素 D 类似物，命名为芽孢杆菌素 DC。评估了芽孢杆菌素 DC 对胶孢炭疽菌的活性。芽孢杆菌素 DC 的中浓度抑制为 1.21μg/mL。此外，芽孢杆菌素 DC 对水生生物的毒性可能较低，其对斑马鱼（Danio rerio）的 96h 半数致死浓度为 22.20μg/mL。我们目前的研究进一步证明，芽孢杆菌素 DC 是一种针对胶孢炭疽菌的有效杀菌剂。
