Tulio Artemio Z, Reese R Neil, Wyzgoski Faith J, Rinaldi Peter L, Fu Ruiling, Scheerens Joseph C, Miller A Raymond
Department of Horticulture and Crop Science, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
J Agric Food Chem. 2008 Mar 26;56(6):1880-8. doi: 10.1021/jf072313k. Epub 2008 Feb 22.
Anthocyanin constituents in black raspberries (Rubus occidentalis L.) were investigated by HPLC-DAD, and their involvement as potent, significant antioxidants in black raspberries was demonstrated by three common antioxidant assays (FRAP, DPPH, ABTS) in this study. Five anthocyanins were present in black raspberries: cyanidin 3-sambubioside, cyanidin 3-glucoside, cyanidin 3-xylosylrutinoside, cyanidin 3-rutinoside, and pelargonidin 3-rutinoside. Their identities and structures, with particular emphasis on cyanidin 3-xylosylrutinoside, were confirmed by NMR spectroscopy. Two of these anthocyanins, cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside, predominated, comprising 24-40 and 49-58%, respectively, of the total anthocyanins in black raspberries. On the basis of both potency and concentration, cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside were found to be the significant contributors to the antioxidant systems of black raspberries. These findings indicate that these two anthocyanin compounds may function as the primary phenolic antioxidants in black raspberries. These two compounds exhibit potential biological activities that may be exploited in conjunction with other naturally occurring bioactive compounds in black raspberry fruit-based products used in clinical trials for the treatment of various types of cancer.
通过高效液相色谱 - 二极管阵列检测器（HPLC - DAD）对黑树莓（Rubus occidentalis L.）中的花青素成分进行了研究，并且在本研究中通过三种常见的抗氧化剂测定方法（铁离子还原抗氧化能力测定法（FRAP）、1,1 - 二苯基 - 2 - 三硝基苯肼（DPPH）、2,2'-联氮 - 双 - 3 - 乙基苯并噻唑啉 - 6 - 磺酸（ABTS））证明了它们作为黑树莓中强效、重要抗氧化剂的作用。黑树莓中存在五种花青素：矢车菊素 - 3 - 桑布双糖苷、矢车菊素 - 3 - 葡萄糖苷、矢车菊素 - 3 - 木糖基芸香糖苷、矢车菊素 - 3 - 芸香糖苷和天竺葵素 - 3 - 芸香糖苷。通过核磁共振光谱（NMR）确定了它们的身份和结构，尤其着重于矢车菊素 - 3 - 木糖基芸香糖苷。这五种花青素中的两种，矢车菊素 - 3 - 芸香糖苷和矢车菊素 - 3 - 木糖基芸香糖苷占主导地位，分别占黑树莓总花青素的24 - 40%和49 - 58%。基于效力和浓度，发现矢车菊素 - 3 - 芸香糖苷和矢车菊素 - 3 - 木糖基芸香糖苷是黑树莓抗氧化系统的重要贡献者。这些发现表明这两种花青素化合物可能作为黑树莓中的主要酚类抗氧化剂发挥作用。这两种化合物具有潜在的生物活性，在用于治疗各类癌症的临床试验的基于黑树莓果实的产品中，可与其他天然存在的生物活性化合物联合利用。
