School of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia.
Institute of Endocrinology and Diabetes, University of Sydney Medical School, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia; Discipline of Paediatrics and Child Health, University of Sydney Medical School, The Children's Hospital at Westmead, Westmead, NSW 2145, Australia.
Food Chem. 2018 Nov 30;267:319-328. doi: 10.1016/j.foodchem.2018.02.111. Epub 2018 Feb 21.
Yellow lupin polysaccharides (YLP-1, YLP-2 and YLP-3) were isolated from the whole seeds of Lupinus luteus L. Their antioxidant activities were evaluated by ABTS and hydroxyl radical scavenging, and Fe chelating assays. Immunostimulatory activities were measured by their ability to activate macrophages to produce TNF-α and NO. Four strains of probiotic bacteria were used to measure their prebiotic activities. YLP-2 with largest galactose content displayed the best activity amongst the three isolated polysaccharides. NMR and FT-IR spectroscopic methods have revealed that YLPs contain galactans and galactomannans which are linked with β-(1,4) glycosidic bond in the main chain. The side chain Galp unit of galactomannan is connected to the main chain Manp by α-(1,6) linkage. The results presented in this paper strongly suggest that YLPs display significant antioxidant, immunostimulatory and prebiotic activities and hence hold great potential as nutraceutical and functional agents.
从羽扇豆属植物（ Lupinus luteus L. ）的全种子中分离得到黄色羽扇豆多糖（YLP-1、YLP-2 和 YLP-3）。通过 ABTS 和羟基自由基清除以及 Fe 螯合测定评估它们的抗氧化活性。通过其激活巨噬细胞产生 TNF-α 和 NO 的能力来测量免疫刺激活性。使用四种益生菌菌株来测量它们的益生元活性。在三种分离的多糖中，半乳糖含量最大的 YLP-2 显示出最佳活性。NMR 和 FT-IR 光谱方法表明 YLPs 含有半乳糖和半乳甘露聚糖，它们在主链中通过β-（1,4）糖苷键连接。半乳甘露聚糖的 Galp 单元侧链通过α-（1,6）键连接到主链 Manp。本文提出的结果强烈表明 YLPs 具有显著的抗氧化、免疫刺激和益生元活性，因此作为营养保健品和功能性制剂具有巨大的潜力。
