Yang Kai, Zhang Yajie, Cai Ming, Guan Rongfa, Neng Jing, Pi Xionge, Sun Peilong
College of Food Science and Technology, Zhejiang University of Technology Hangzhou 310014 P. R. China
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Science Hangzhou 310021 P. R. China.
RSC Adv. 2020 Apr 14;10(25):14794-14802. doi: 10.1039/c9ra10798c. eCollection 2020 Apr 8.
Until recently, a variety of oligosaccharides from fruits, vegetables and mushrooms have demonstrated positive prebiotic effects. , a well-known traditional medicine and tonic in East Asia, has been utilized in the prevention and treatment of a broad range of illnesses. In this study, each of three oligosaccharides was obtained from the polysaccharide extraction by-products of sporoderm: the unbroken and broken spores of (UB-GLS, B-GLS). Their molecular weight distribution, monosaccharide composition and preliminary structures were analyzed using gel permeation chromatography (GPC), GC-MS, UV and FTIR, respectively. All of the oligosaccharides were found to exhibit prebiotic activities, evaluated by detecting growth stimulation on . Among these, UB-O80 and B-O80 displayed the most significant effects ( < 0.05) in these groups, and UB-O80 showed higher resistance to hydrolysis by artificial human gastric juice compared with inulin, giving a maximum hydrolysis rate of 1.65%. Compared with inulin media, also revealed high tolerance to lower pH levels and simulated gastric juices in UB-O80 and B-O80 media. Compared with a control in gut microbiota fermentation, the abundance of some beneficial bacteria increased and some harmful bacteria declined in the groups of UB-O80 and B-O80. In conclusion, the results suggest that GLS oligosaccharides could be exploited as promising prebiotics for the enhancement of human health.
直到最近，来自水果、蔬菜和蘑菇的多种低聚糖已显示出积极的益生元效应。在东亚，作为一种著名的传统药物和滋补品，已被用于预防和治疗多种疾病。在本研究中，三种低聚糖分别从灵芝孢子粉多糖提取副产物中获得：完整和破碎的灵芝孢子（UB-GLS、B-GLS）。分别使用凝胶渗透色谱法（GPC）、气相色谱-质谱联用仪（GC-MS）、紫外光谱仪（UV）和傅里叶变换红外光谱仪（FTIR）分析了它们的分子量分布、单糖组成和初步结构。通过检测对嗜酸乳杆菌的生长刺激来评估，发现所有低聚糖均表现出益生元活性。其中，UB-O80和B-O80在这些组中显示出最显著的效果（P<0.05），并且与菊粉相比，UB-O80对人工胃液水解的耐受性更高，最大水解率为1.65%。与菊粉培养基相比，在UB-O80和B-O80培养基中，嗜酸乳杆菌对较低pH值和模拟胃液也显示出高耐受性。与肠道微生物群发酵中的对照相比，UB-O80和B-O80组中一些有益细菌的丰度增加，一些有害细菌的丰度下降。总之，结果表明灵芝低聚糖有望作为益生元用于增进人类健康。
