125I标记的人肿瘤坏死因子（LuKII）与特定细胞表面受体的高亲和力结合。
High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors.
作者信息
Rubin B Y, Anderson S L, Sullivan S A, Williamson B D, Carswell E A, Old L J
出版信息
J Exp Med. 1985 Sep 1;162(3):1099-104. doi: 10.1084/jem.162.3.1099.
Abstract
125I-labeled TNF(LuKII) (tumor necrosis factor) binds specifically to human and mouse cell lines sensitive to the cytotoxic effect of TNF, but not to cells made resistant to TNF. TNF-sensitive cells have cell surface receptors with a high affinity for TNF(LuKII). Mouse TNF competes with TNF(LuKII) for receptor binding. Scatchard analysis of the binding data yielded linear plots and suggests that TNF(LuKII) binds to homogeneous receptor sites. The number of TNF(LuKII) receptors on two TNF-sensitive cell lines is 200-300 per cell and the affinity constant of the receptor for TNF(LuKII) is approximately 1 X 10(-10) M.
摘要
125I标记的肿瘤坏死因子（LuKII）特异性结合对肿瘤坏死因子细胞毒性作用敏感的人源和鼠源细胞系，但不结合对肿瘤坏死因子产生抗性的细胞。对肿瘤坏死因子敏感的细胞具有对肿瘤坏死因子（LuKII）具有高亲和力的细胞表面受体。小鼠肿瘤坏死因子与肿瘤坏死因子（LuKII）竞争受体结合。对结合数据进行Scatchard分析得到线性图，表明肿瘤坏死因子（LuKII）结合到同质受体位点。两个对肿瘤坏死因子敏感的细胞系上肿瘤坏死因子（LuKII）受体的数量为每个细胞200 - 300个，受体对肿瘤坏死因子（LuKII）的亲和常数约为1×10（-10）M。
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