具有肿瘤坏死活性的淋巴因子——人淋巴毒素cDNA的克隆与表达
Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity.
作者信息
Gray P W, Aggarwal B B, Benton C V, Bringman T S, Henzel W J, Jarrett J A, Leung D W, Moffat B, Ng P, Svedersky L P
出版信息
Nature. 1984;312(5996):721-4. doi: 10.1038/312721a0.
DOI:10.1038/312721a0
PMID:6334807
Abstract
A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.
摘要
编码人淋巴毒素的化学合成基因和天然互补DNA被分离出来，并进行工程改造以便在大肠杆菌中表达。纯化的重组淋巴毒素在体外对小鼠和人类肿瘤细胞系显示出细胞毒性活性，并在体内导致某些小鼠肉瘤坏死。
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