Hu Ke, Li Leina, Liao Yaping, Liang Meng
Department of Biological Sciences, Bengbu Medical College, Bengbu, Anhui, People's Republic of China.
Genes Genomics. 2018 Jul;40(7):781-787. doi: 10.1007/s13258-018-0690-4. Epub 2018 Apr 10.
Spermatogenesis is a process which includes the following phases: spermatogonial stem cell proliferation and differentiation, spermatogonia, spermatocyte, spermatid and mature sperm. Spermatogenic failure is the important factor resulting in male infertility. Recent studies showed that long noncoding RNA (lncRNA) have been found to be involved in the regulation of male reproduction. However, lncRNA associated with spermatogenesis and their mechanisms of action are unclear. The aim of this study is to explore the role and molecular mechanism of lncRNA in spermatogenesis. LncRNA microarray of germ cells and bioinformatic analysis showed lncRNA Gm2044 may play potential roles in spermatogenesis. The expression level of RNA and protein were analyzed by RT-qPCR and western blotting, respectively. The interaction of lncRNA with mRNA was detected by RNA pull down and cellular proliferation was measured using CCK-8 reagent. Testis-enriched lncRNA Gm2044 is abundant in mouse spermatocytes. Gm2044 can suppress the translation of adjacent spermatogenesis-related gene Utf1 by interacting with Utf1 mRNA. Furthermore, the proliferation of mouse spermatogonia GC-1 cell line and spermatocyte GC-2 cell line was inhibited by Gm2044.
LncRNA Gm2044 was identified to inhibit Utf1 mRNA translation and play important roles in spermatogenesis.
精子发生是一个包括以下阶段的过程：精原干细胞增殖与分化、精原细胞、精母细胞、精子细胞和成熟精子。精子发生失败是导致男性不育的重要因素。最近的研究表明，长链非编码RNA（lncRNA）参与男性生殖的调控。然而，与精子发生相关的lncRNA及其作用机制尚不清楚。本研究的目的是探讨lncRNA在精子发生中的作用及其分子机制。生殖细胞lncRNA芯片及生物信息学分析表明，lncRNA Gm2044可能在精子发生中发挥潜在作用。分别通过RT-qPCR和蛋白质印迹分析RNA和蛋白质的表达水平。通过RNA下拉检测lncRNA与mRNA的相互作用，并使用CCK-8试剂测量细胞增殖。睾丸富集lncRNA Gm2044在小鼠精母细胞中丰富。Gm2044可通过与Utf1 mRNA相互作用抑制相邻精子发生相关基因Utf1的翻译。此外，Gm2044抑制小鼠精原细胞GC-1细胞系和精母细胞GC-2细胞系的增殖。
lncRNA Gm2044被鉴定为抑制Utf1 mRNA翻译并在精子发生中发挥重要作用。
