Snowden B W, Halliburton I W
J Gen Virol. 1985 Sep;66 ( Pt 9):2039-44. doi: 10.1099/0022-1317-66-9-2039.
Monospecific rabbit antisera against purified herpes simplex virus type 1 (HSV-1) gB, gC or gD antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (EHV-1)-infected cells was used in Western blotting to identify antigenically related proteins in cells infected with HSV-1, HSV-2, bovine mammillitis virus or EHV-1. Monomeric and oligomeric polypeptides related to HSV-1 gB were found in cells infected with each of the four herpesviruses. The gC antiserum was specific for HSV-1 and the gD antiserum reacted only with polypeptides in HSV-1- or HSV-2-infected cells. Neither gC nor gD antiserum revealed the existence of oligomeric forms of the glycoproteins with the single exception of HSV-1 strain KOS which had a heat-unstable high molecular weight form gD. The EHV-1 antiserum reacted with high molecular weight polypeptides of the same mobility as the oligomeric form of gB in cells infected with each of the four herpesviruses.
针对纯化的单纯疱疹病毒1型（HSV-1）gB、gC或gD抗原的单特异性兔抗血清，或针对感染1型马疱疹病毒（EHV-1）细胞的多价兔抗血清，用于蛋白质印迹法，以鉴定感染HSV-1、HSV-2、牛乳头炎病毒或EHV-1的细胞中抗原相关蛋白。在感染四种疱疹病毒中的每一种的细胞中都发现了与HSV-1 gB相关的单体和寡聚多肽。gC抗血清对HSV-1具有特异性，gD抗血清仅与感染HSV-1或HSV-2的细胞中的多肽发生反应。除了具有热不稳定高分子量形式gD的HSV-1毒株KOS外，gC和gD抗血清均未显示糖蛋白寡聚体形式的存在。EHV-1抗血清与感染四种疱疹病毒中的每一种的细胞中与gB寡聚体形式迁移率相同的高分子量多肽发生反应。
