Balachandran N, Oba D E, Hutt-Fletcher L M
J Virol. 1987 Apr;61(4):1125-35. doi: 10.1128/JVI.61.4.1125-1135.1987.
Polyvalent rabbit antisera against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2), cytomegalovirus (CMV), and Epstein-Barr virus (EBV), monospecific antisera against affinity-purified HSV-2 glycoproteins gB and gG, and a panel of monoclonal antibodies against HSV and EBV proteins were used to analyze cross-reactive molecules in cells infected with the four herpesviruses. A combination of immunoprecipitation and Western blotting with these reagents was used to determine that all four viruses coded for a glycoprotein that cross-reacted with HSV-1 gB. CMV coded for proteins that cross-reacted with HSV-2 gC, gD, and gE. Both CMV and EBV coded for proteins that cross-reacted with HSV-2 gG. Antigenic counterparts to the p45 nucleocapsid protein of HSV-2 were present in HSV-1 and CMV, and counterparts of the major DNA-binding protein and the ribonucleotide reductase of HSV-1 were present in all the viruses. The EBV virion glycoprotein gp85 was immunoprecipitated by antisera to HSV-1, HSV-2, and CMV. Antisera to CMV and EBV neutralized the infectivity of both HSV-1 and HSV-2 at high concentrations. This suggests that cross-reactivity between these four human herpesviruses may have pathogenic as well as evolutionary significance.
使用针对1型和2型单纯疱疹病毒（HSV - 1和HSV - 2）、巨细胞病毒（CMV）和EB病毒（EBV）的多价兔抗血清，针对亲和纯化的HSV - 2糖蛋白gB和gG的单特异性抗血清，以及一组针对HSV和EBV蛋白的单克隆抗体，来分析感染这四种疱疹病毒的细胞中的交叉反应分子。使用这些试剂通过免疫沉淀和蛋白质印迹相结合的方法确定，所有四种病毒都编码一种与HSV - 1 gB发生交叉反应的糖蛋白。CMV编码的蛋白与HSV - 2 gC、gD和gE发生交叉反应。CMV和EBV都编码与HSV - 2 gG发生交叉反应的蛋白。HSV - 2的p45核衣壳蛋白的抗原对应物存在于HSV - 1和CMV中，HSV - 1的主要DNA结合蛋白和核糖核苷酸还原酶的对应物存在于所有病毒中。EBV病毒体糖蛋白gp85可被针对HSV - 1、HSV - 2和CMV的抗血清免疫沉淀。针对CMV和EBV的抗血清在高浓度时可中和HSV - 1和HSV - 2的感染性。这表明这四种人类疱疹病毒之间的交叉反应可能具有致病以及进化意义。
