Bridges C G, Ledger N, Edington N
Department of Microbiology and Parasitology, Royal Veterinary College, London, U.K.
Immunology. 1988 Feb;63(2):193-8.
Ponies, without evidence of previous exposure to Equine herpes virus-1 (EHV-1), were experimentally infected with EHV-1 subtype 2 and investigated for lymphocyte transformation to virus-infected cell polypeptides, as shown by separation with gel electrophoresis. Animals made significant responses to Western blot fractions that corresponded to molecular weights of approximately 30,000, 40,000-45,000, 60,000-65,000, 80,000-95,000 and 100,000-140,000 MW. These molecular weight ranges correlated with the positions of major EHV-1 subtype 2 glycoproteins that were found at migration distances approximating to 137,000, 111,000, 90,000, 65,000 and 47,000 MW. Responses were also made to a subset of similar points on the subtype 1 profile. Hyperimmune equine serum precipitated numerous infected-cell proteins of both subtypes; in particular the recognition of polypeptides with MW of 142,000, 132,000, 114,000, and 46,000 was in agreement with the mitogenic responses. Labelling with 125I indicated that immunoprecipitated greater than 250,000, 182,000, 142,000, 132,000, 75,000, 46,000 and 32,000/34,000 MW products were exposed on the surface of infected cells.
对未曾接触过马疱疹病毒1型（EHV-1）的小马，用EHV-1 2型亚型进行实验性感染，并通过凝胶电泳分离来研究淋巴细胞对病毒感染细胞多肽的转化情况。动物对与分子量约为30,000、40,000 - 45,000、60,000 - 65,000、80,000 - 95,000和100,000 - 140,000道尔顿相对应的蛋白质印迹条带产生了显著反应。这些分子量范围与主要的EHV-1 2型亚型糖蛋白的位置相关，这些糖蛋白在迁移距离近似于137,000、111,000、90,000、65,000和47,000道尔顿处被发现。对1型亚型图谱上的一组相似点也有反应。超免疫马血清沉淀出了两种亚型的许多感染细胞蛋白；特别是对分子量为142,000、132,000、114,000和46,000的多肽的识别与促有丝分裂反应一致。用125I标记表明，免疫沉淀出的大于250,000、182,000、142,000、132,000、75,000、46,000和32,000/34,000道尔顿的产物暴露在感染细胞表面。
