Stålhandske P, Hyypiä T, Allard A, Halonen P, Pettersson U
J Med Virol. 1985 Jul;16(3):213-8. doi: 10.1002/jmv.1890160302.
Nucleic acid hybridization was used for the detection of adenovirus DNA in stool specimens, and the results were compared with those obtained by a radioimmunoassay (RIA) for adenovirus hexon antigen. DNA from 40 specimens, 18 of which were positive by RIA, were spotted onto nitrocellulose filters and analyzed by hybridization using radioactively labeled adenovirus-2 DNA or a cloned DNA fragment from enteric adenovirus-41 as probes. With the adenovirus-2 DNA probe, 15 of the 18 RIA-positive specimens were also positive in the hybridization assay, and one of the RIA negative specimens was also scored as positive. The cloned adenovirus-41 fragment gave a positive signal with five specimens, all of which were also detected with the adenovirus-2 DNA probe. The results show that hybridization is an alternative method for detection of adenovirus in stool specimens. The sensitivity of the assay is comparable to that of the RIA.
采用核酸杂交法检测粪便标本中的腺病毒DNA，并将结果与腺病毒六邻体抗原放射免疫测定（RIA）的结果进行比较。将来自40份标本的DNA（其中18份经RIA检测为阳性）点样到硝酸纤维素滤膜上，并用放射性标记的腺病毒2型DNA或来自肠道腺病毒41型的克隆DNA片段作为探针进行杂交分析。使用腺病毒2型DNA探针时，18份RIA阳性标本中的15份在杂交试验中也呈阳性，1份RIA阴性标本也被判定为阳性。克隆的腺病毒41型片段在5份标本中给出阳性信号，所有这些标本也能用腺病毒2型DNA探针检测到。结果表明，杂交法是检测粪便标本中腺病毒的另一种方法。该检测方法的灵敏度与RIA相当。
