Takiff H E, Seidlin M, Krause P, Rooney J, Brandt C, Rodriguez W, Yolken R, Straus S E
J Med Virol. 1985 Jun;16(2):107-18. doi: 10.1002/jmv.1890160203.
Enteric adenoviruses (EAds) (candidate adenoviruses 40 and 41, subgroups F and G) have been implicated in the etiology of gastroenteritis in infants, but their clinical significance has been unclear because a rapid test to distinguish these agents from other adenovirus (Ad) types has not been available. We developed a dot-blot hybridization assay for EAd DNA using a cloned DNA fragment that has little homology to non-EAd DNAs. The dot-blot system detected less than 20 pg of EAd DNA, while showing minimal cross hybridization to representative strains from all other Ad groups. There was no detectable hybridization to extracts of samples known to contain other enteric viruses. It was further shown that low levels of EAds in specimens could be amplified by culturing for 1 day in 293 cells. Stool samples and tissue culture lysates prescreened by electron microscopy, cell culture or ELISA were tested in a blind fashion. Using endonuclease analysis as the standard for typing the isolates, we found the dot-blot system to have a 91% sensitivity and 71% specificity for detecting EAds and distinguishing them from other Ads. False-positive and equivocal dot-blot results appeared to be caused by other Ads.
肠道腺病毒（EAds）（候选腺病毒40型和41型，F和G亚组）被认为与婴儿肠胃炎的病因有关，但由于缺乏一种能将这些病原体与其他腺病毒（Ad）类型区分开来的快速检测方法，其临床意义一直不明确。我们利用一个与非EAd DNA几乎没有同源性的克隆DNA片段，开发了一种用于检测EAd DNA的斑点杂交试验。该斑点杂交系统能检测到少于20 pg的EAd DNA，同时与所有其他Ad组的代表性菌株的交叉杂交最少。与已知含有其他肠道病毒的样本提取物没有可检测到的杂交。进一步研究表明，通过在293细胞中培养1天，可以扩增标本中低水平的EAds。对经电子显微镜、细胞培养或ELISA预筛选的粪便样本和组织培养裂解物进行盲法检测。以核酸内切酶分析作为分型分离株的标准，我们发现斑点杂交系统检测EAds并将其与其他Ads区分开来的灵敏度为91%，特异性为71%。假阳性和模糊的斑点杂交结果似乎是由其他Ads引起的。
