从猪和人类中分离出的产超广谱β-内酰胺酶的 Aerogenes 肠杆菌的基因组序列草案。
Draft genome sequences of extended-spectrum β-lactamase-producing Enterobacter aerogenes isolated from swine and human.
机构信息
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Food Safety and Environmental Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Clinical Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
出版信息
J Glob Antimicrob Resist. 2018 Sep;14:70-71. doi: 10.1016/j.jgar.2018.06.006. Epub 2018 Jun 20.
OBJECTIVES
The draft genome sequences of two Enterobacter aerogenes strains (HN503E2II and PN108E5IIB) isolated from two Cameroonian abattoirs are reported.
METHODS
Bacterial genomic DNA of the two isolates was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using CLC Genomics Workbench and SPAdes. The assembled contigs were annotated, and antibiotic resistance genes, virulence factors and plasmids were identified.
RESULTS
Whole-genome sequencing revealed that both strains were similar, with genomes of 4878638bp and 4794257bp, encoding several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, fosfomycin, phenicols, sulphonamides, trimethoprim, macrolides and tetracycline. In silico analysis also revealed chromosomal integration of one plasmid in the genome of PN108E5IIB.
CONCLUSION
The genome sequences reported here will provide useful information for a better understanding of the genetic structure of E. aerogenes in Africa.
目的
报告从喀麦隆两个屠宰场分离的两株阴沟肠杆菌（HN503E2II 和 PN108E5IIB）的基因组草案序列。
方法
使用 Illumina MiSeq 平台对这两种分离株的细菌基因组 DNA 进行测序。使用 CLC Genomics Workbench 和 SPAdes 对生成的读数进行从头组装。对组装的 contigs 进行注释，并鉴定抗生素抗性基因、毒力因子和质粒。
结果
全基因组测序表明，这两个菌株非常相似，基因组分别为 4878638bp 和 4794257bp，编码与β-内酰胺类、氟喹诺酮类、氨基糖苷类、磷霉素、苯氧青霉素类、磺胺类、甲氧嘧啶、大环内酯类和四环素类耐药相关的几种抗性基因。计算机分析还显示，PN108E5IIB 基因组中有一个质粒的染色体整合。
结论
本报告的基因组序列将为更好地了解非洲阴沟肠杆菌的遗传结构提供有用信息。
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