Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Food Safety and Environmental Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa; Department of Clinical Microbiology, Centre of Expertise and Biological Diagnostic of Cameroon (CEDBCAM), Yaoundé, Cameroon.
J Glob Antimicrob Resist. 2018 Sep;14:68-69. doi: 10.1016/j.jgar.2018.06.004. Epub 2018 Jun 19.
Here we report the draft genome sequence of Escherichia coli strain HN503E1II isolated from a nasal sample of an abattoir worker in Cameroon.
Bacterial genomic DNA of E. coli HN503E1II was sequenced using an Illumina MiSeq platform. Generated reads were de novo assembled using the Qiagen CLC Genomics Workbench. The assembled contigs were annotated and antibiotic resistance genes, virulence factors, plasmids and the sequence type (ST) were identified.
The genome comprised a circular chromosome of 4 674201bp, with a 50.78% G+C content and several resistance genes associated with resistance to β-lactams, fluoroquinolones, aminoglycosides, sulphonamides, trimethoprim, macrolides and tetracycline. The isolate was assigned to ST10 with 100% identity among the seven housekeeping genes. In silico analysis also revealed the presence six plasmid types and one virulence factor.
The genome sequence reported here will provide valuable information for a better understanding of the genetic structure of the E. coli genome in Africa.
本研究报告了从喀麦隆一名屠宰场工人的鼻腔样本中分离的大肠杆菌 HN503E1II 菌株的基因组草图序列。
使用 Illumina MiSeq 平台对大肠杆菌 HN503E1II 的细菌基因组 DNA 进行测序。使用 Qiagen CLC Genomics Workbench 对生成的reads 进行从头组装。对组装的 contigs 进行注释，并鉴定抗生素耐药基因、毒力因子、质粒和序列型（ST）。
基因组由一个 4674201bp 的环状染色体组成，G+C 含量为 50.78%，并携带多个与β-内酰胺类、氟喹诺酮类、氨基糖苷类、磺胺类、甲氧苄啶、大环内酯类和四环素类耐药相关的耐药基因。该分离株被分配到 ST10，7 个看家基因的同一性为 100%。计算机分析还显示存在六种质粒类型和一种毒力因子。
本研究报告的基因组序列将为更好地了解非洲大肠杆菌基因组的遗传结构提供有价值的信息。
