黏膜接种灭活牛分枝杆菌对斑马鱼（Danio rerio）分枝杆菌病的控制。
Control of mycobacteriosis in zebrafish (Danio rerio) mucosally vaccinated with heat-inactivated Mycobacterium bovis.
机构信息
SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain.
SaBio, Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
出版信息
Vaccine. 2018 Jul 16;36(30):4447-4453. doi: 10.1016/j.vaccine.2018.06.042. Epub 2018 Jun 21.
BACKGROUND
Mycobacterial infections greatly affect human and animal health worldwide, and vaccines are effective, sustainable and economic interventions for the prevention and control of these infectious diseases. Recent results support the use of zebrafish as a model for studying the pathophysiology of mycobacterial infection and for the development of novel interventions for tuberculosis (TB) control. Recently, we showed that oral immunization with the heat-inactivated M. bovis vaccine (M. bovis IV) protect wild boar against TB, and suggested that this vaccine may controls mycobacterial infection in other species.
METHODS
In this study we evaluated the effect of M. bovis IV on the control of mycobacteriosis in zebrafish mucosally vaccinated by immersion and challenged intraperitoneally with Mycobacterium marinum.
RESULTS
The results showed that the M. bovis IV administered by immersion protected zebrafish against mycobacteriosis caused by M. marinum by reduction in mycobacterial infection, the number of mycobacteria per granuloma and the number of granulomas per fish. An IgM antibody response against M. bovis antigens was developed in vaccinated fish. Evidences suggested that the protective mechanism elicited by mucosal vaccination with M. bovis IV in zebrafish was based on the activation of the innate immune response through the C3 pathway.
CONCLUSIONS
These results support the use of the M. bovis IV administered by immersion for the control of mycobacteriosis in fish.
背景
分枝杆菌感染极大地影响了全球人类和动物的健康，疫苗是预防和控制这些传染病的有效、可持续和经济的干预措施。最近的研究结果支持使用斑马鱼作为研究分枝杆菌感染病理生理学的模型，并为结核病（TB）控制开发新的干预措施。最近，我们发现，用灭活的牛分枝杆菌疫苗（M. bovis IV）进行口服免疫可保护野猪免受结核病的侵害，并表明该疫苗可能控制其他物种的分枝杆菌感染。
方法
在这项研究中，我们评估了 M. bovis IV 通过浸泡免疫接种斑马鱼并通过腹腔内 challenged 感染海洋分枝杆菌（Mycobacterium marinum）对控制鱼类分枝杆菌病的影响。
结果
结果表明，通过浸泡给予的 M. bovis IV 通过减少分枝杆菌感染、每个肉芽肿中的分枝杆菌数量和每条鱼中的肉芽肿数量，保护斑马鱼免受由海洋分枝杆菌引起的分枝杆菌病。接种鱼体内产生了针对 M. bovis 抗原的 IgM 抗体反应。有证据表明，M. bovis IV 通过粘膜免疫接种在斑马鱼中引发的保护机制是通过 C3 途径激活先天免疫反应。
结论
这些结果支持使用浸泡给予的 M. bovis IV 来控制鱼类分枝杆菌病。
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