Molecular and Cellular Modeling Group, Heidelberg Institute for Theoretical Studies (HITS), 69118 Heidelberg, Germany; The Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (HBIGS), Heidelberg University, 69120 Heidelberg, Germany.
Computational Structural Biology Laboratory, Department of Cellular and Developmental Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany; Center for Multiscale Theory and Computation, Westfälische Wilhelms University, 48149 Münster, Germany.
Structure. 2018 Aug 7;26(8):1050-1057. doi: 10.1016/j.str.2018.05.009. Epub 2018 Jun 21.
There is renewed interest in linker histone (LH)-nucleosome binding and how LHs influence eukaryotic DNA compaction. For a long time, the goal was to uncover "the structure of the chromatosome," but recent studies of LH-nucleosome complexes have revealed an ensemble of structures. Notably, the reconstituted LH-nucleosome complexes used in experiments rarely correspond to the sequence combinations present in organisms. For a full understanding of the determinants of the distribution of the chromatosome structural ensemble, studies must include a complete description of the sequences and experimental conditions used, and be designed to enable systematic evaluation of sequence and environmental effects.
人们对连接组蛋白 (LH)-核小体结合以及 LH 如何影响真核生物 DNA 压缩重新产生了兴趣。长期以来，人们的目标是揭示“染色质小体的结构”，但最近对 LH-核小体复合物的研究揭示了一系列结构。值得注意的是，实验中使用的重建 LH-核小体复合物很少与生物体中存在的序列组合相对应。为了全面了解染色质小体结构组合分布的决定因素，研究必须包括对所使用的序列和实验条件的完整描述，并设计成能够系统地评估序列和环境效应。
