Mazoochian Leila, Mohammad Sadeghi Hamid Mir, Pourfarzam Morteza
Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Biotechnology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
J Res Med Sci. 2018 May 30;23:47. doi: 10.4103/jrms.JRMS_961_17. eCollection 2018.
In this study, we investigated the associations of erythrocytes fatty acid composition, activities of delta-5 desaturase (D5D) and delta-6 desaturase (D6D), and other metabolic risk factors, with type 2 diabetes (T2D) risk to determine if rs174583 polymorphism of FADS2 gene had any effect on these associations.
Fatty acid profile of erythrocytes was determined using gas chromatography-mass spectrometry in 95 T2D patients and 95 apparently healthy participants. The genotypes of single-nucleotide polymorphism (SNP) of FADS2 gene were determined using the polymerase chain reaction-restriction fragment length polymorphism technique. Other biochemical parameters were measured in the serum using standard analytical procedures.
D6D activity was increased ( < 0.001) and D5D activity was decreased in T2D patients ( < 0.001) compared to controls. Homeostatic model assessment insulin resistance (HOMA-IR) index was positively correlated with D6D ( = 0.34, < 0.001) and negatively correlated with D5D ( = -0.19, = 0.02). Palmitic acid ( < 0.001) and dihomo-gamma-linolenic acid ( = 0.03) were higher and linoleic acid ( < 0.001) and arachidonic acid (AA) ( < 0.001) were lower in T2D patients. The distribution of rs174583 genotypes which includes C/T, C/C, and T/T was not different in the two groups ( = 0.63).
In the population studied, there was a strong association in the erythrocytes fatty acid composition, D5D and D6D activities and other metabolic risk factors between non-T2D and T2D patients. In addition, there was a strong association in erythrocytes DGLA and AA contents and D5D activities between rs174583 genotypes in all participants. However, the distribution of rs174583 genotypes did not differ significantly between T2D patient and controls, and it did not appear to be an association between rs174583 SNP and incident of T2D.
在本研究中，我们调查了红细胞脂肪酸组成、δ-5 去饱和酶（D5D）和δ-6 去饱和酶（D6D）的活性以及其他代谢风险因素与 2 型糖尿病（T2D）风险之间的关联，以确定 FADS2 基因的 rs174583 多态性是否对这些关联有任何影响。
采用气相色谱 - 质谱法测定 95 例 T2D 患者和 95 例明显健康参与者的红细胞脂肪酸谱。使用聚合酶链反应 - 限制性片段长度多态性技术测定 FADS2 基因单核苷酸多态性（SNP）的基因型。使用标准分析程序测定血清中的其他生化参数。
与对照组相比，T2D 患者的 D6D 活性增加（<0.001），D5D 活性降低（<0.001）。稳态模型评估胰岛素抵抗（HOMA - IR）指数与 D6D 呈正相关（=0.34，<0.001），与 D5D 呈负相关（=-0.19，=0.02）。T2D 患者的棕榈酸（<0.001）和二高 - γ - 亚麻酸（=0.03）含量较高，亚油酸（<0.001）和花生四烯酸（AA）（<0.001）含量较低。两组中包括 C/T、C/C 和 T/T 的 rs174583 基因型分布无差异（=0.63）。
在所研究的人群中，非 T2D 患者和 T2D 患者在红细胞脂肪酸组成、D5D 和 D6D 活性以及其他代谢风险因素之间存在很强的关联。此外，在所有参与者中，rs174583 基因型之间的红细胞二高 - γ - 亚麻酸和 AA 含量以及 D5D 活性之间存在很强的关联。然而，T2D 患者和对照组之间 rs174583 基因型的分布没有显著差异，并且 rs174583 SNP 与 T2D 的发病似乎没有关联。
