Daniels S B, Strausbaugh L D, Armstrong R A
Mol Gen Genet. 1985;200(2):258-65. doi: 10.1007/BF00425433.
In this report we describe the successful transformation of Drosophila simulans with an autonomous P element from Drosophila melanogaster without the use of a selectable marker. This result demonstrates that there is no species barrier for P element transposition. Utilizing gel blotting and in situ hybridization techniques, we have monitored the behavior of newly-introduced P elements in several D. simulans transformed lines over twelve generations. In most instances, an overall increase in the number of P elements was observed. An examination of the frequency of P-element-bearing individuals in one line revealed the rapid spread of P elements through the population. Analysis of well-characterized sublines confirmed that P elements increase in number by transposition to new genomic sites. The formation of degenerate elements occurred in at least one case. These observations suggest that P elements may behave similarly in D. melanogaster and D. simulans.
在本报告中，我们描述了在不使用选择标记的情况下，利用来自黑腹果蝇的自主P因子成功转化拟果蝇的过程。这一结果表明，P因子转座不存在物种障碍。利用凝胶印迹和原位杂交技术，我们在12代中监测了几个转化的拟果蝇品系中新引入的P因子的行为。在大多数情况下，观察到P因子数量总体增加。对一个品系中携带P因子个体的频率进行检查发现，P因子在群体中迅速传播。对特征明确的亚系进行分析证实，P因子通过转座到新的基因组位点而增加数量。至少在一个案例中发生了退化元件的形成。这些观察结果表明，P因子在黑腹果蝇和拟果蝇中的行为可能相似。
