Carareto C M, Kim W, Wojciechowski M F, O'Grady P, Prokchorova A V, Silva J C, Kidwell M G
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson 85721, USA.
Genetica. 1997;101(1):13-33. doi: 10.1023/a:1018339603370.
The use of transposable elements (TEs) as genetic drive mechanisms was explored using Drosophila melanogaster as a model system. Alternative strategies, employing autonomous and nonautonomous P element constructs were compared for their efficiency in driving the ry+ allele into populations homozygous for a ry- allele at the genomic rosy locus. Transformed flies were introduced at 1%, 5%, and 10% starting frequencies to establish a series of populations that were monitored over the course of 40 generations, using both phenotypic and molecular assays. The transposon-borne ry+ marker allele spread rapidly in almost all populations when introduced at 5% and 10% seed frequencies, but 1% introductions frequently failed to become established. A similar initial rapid increase in frequency of the ry+ transposon occurred in several control populations lacking a source of transposase. Constructs carrying ry+ markers also increased to moderate frequencies in the absence of selection on the marker. The results of Southern and in situ hybridization studies indicated a strong inverse relationship between the degree of conservation of construct integrity and transposition frequency. These finding have relevance to possible future applications of transposons as genetic drive mechanisms.
以黑腹果蝇作为模型系统，对转座元件（TEs）作为基因驱动机制的应用进行了探索。比较了采用自主型和非自主型P元件构建体的替代策略，以驱动ry + 等位基因进入基因组中 rosy 位点上ry - 等位基因纯合的群体，其效率如何。将转化果蝇以1%、5%和10%的起始频率引入，以建立一系列群体，并在40代的过程中使用表型和分子检测方法进行监测。当以5%和10%的起始频率引入时，转座子携带的ry + 标记等位基因在几乎所有群体中迅速传播，但以1%的频率引入时常常无法建立起来。在几个缺乏转座酶来源的对照群体中，ry + 转座子的频率也出现了类似的初始快速增加。在没有对标记进行选择的情况下，携带ry + 标记的构建体频率也增加到了中等水平。Southern杂交和原位杂交研究结果表明，构建体完整性的保守程度与转座频率之间存在强烈的负相关关系。这些发现与转座子未来作为基因驱动机制的可能应用相关。
