在拉瓦尔品第（巴基斯坦）肾脏病中心流行的产碳青霉烯酶的生物体和先进的基于分子微阵列的碳青霉烯酶检测方法的评估。
Prevalence of carbapenemase-producing organisms at the Kidney Center of Rawalpindi (Pakistan) and evaluation of an advanced molecular microarray-based carbapenemase assay.
机构信息
Research & Development, Abbott (Alere Technologies GmbH), Jena, Germany.
InfectoGnostics Research Campus, Jena, Germany.
出版信息
Future Microbiol. 2018 Sep;13:1225-1246. doi: 10.2217/fmb-2018-0082. Epub 2018 Jun 25.
AIM
A DNA microarray-based assay for the detection of antimicrobial resistance (AMR) genes was used to study carbapenemase-producing organisms at the Kidney Center of Rawalpindi, Pakistan.
METHODS
The evaluation of this assay was performed using 97 reference strains with confirmed AMR genes. Testing of 7857 clinical samples identified 425 Gram-negative bacteria out of which 82 appeared carbapenem resistant. These isolates were analyzed using VITEK-2 for phenotyping and the described AMR assay for genotyping.
RESULTS
The most prevalent carbapenemase gene was blaNDM and in 12 isolates we detected two carbapenemase genes (e.g., blaNDM/blaOXA-48).
CONCLUSION
Our prevalence data from Pakistan show that - as in other parts of the world - carbapenemase-producing organisms with different underlying resistance mechanisms are emerging, and this warrants intensified and constant surveillance.
目的
本研究使用基于 DNA 微阵列的检测方法，检测了巴基斯坦拉瓦尔品第市肾脏病中心的耐抗生素（AMR）基因的产生菌。
方法
使用 97 株经证实具有 AMR 基因的参考菌株对该检测方法进行了评估。对 7857 份临床样本进行检测，从中鉴定出 425 株革兰氏阴性菌，其中 82 株表现出对碳青霉烯类药物的耐药性。采用 VITEK-2 对这些分离株进行表型分析，同时采用描述的 AMR 检测方法进行基因分型。
结果
最常见的碳青霉烯酶基因是 blaNDM，在 12 株分离株中检测到两种碳青霉烯酶基因（如 blaNDM/blaOXA-48）。
结论
我们从巴基斯坦获得的流行数据表明，与世界其他地区一样，具有不同潜在耐药机制的碳青霉烯酶产生菌正在出现，这需要加强和持续监测。
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