乳酸乳球菌温和噬菌体BK5-T的自发缺失突变体及BK5-T附着位点的定位
Spontaneous deletion mutants of the Lactococcus lactis temperate bacteriophage BK5-T and localization of the BK5-T attP site.
作者信息
Boyce J D, Davidson B E, Hillier A J
机构信息
Russell Grimwade School of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, Australia.
出版信息
Appl Environ Microbiol. 1995 Nov;61(11):4105-9. doi: 10.1128/aem.61.11.4105-4109.1995.
Abstract
Spontaneous deletion mutants of the temperate lactococcal bacteriophage BK5-T were obtained when the phage was grown vegetatively on the indicator strain Lactococcus lactis subsp. cremoris H2. One deletion mutant was unable to form stable lysogens, and analysis of this mutant led to the identification of the BK5-T attP site and the integrase gene (int). The core sequences of the BK5-T attP and host attB regions are conserved in a number of lactococcal phages and L. lactis strains.
摘要
当温和型乳酸乳球菌噬菌体BK5-T在指示菌株乳酸乳球菌乳脂亚种H2上进行营养生长时，获得了其自发缺失突变体。其中一个缺失突变体无法形成稳定的溶原菌，对该突变体的分析导致了BK5-T附着位点（attP）和整合酶基因（int）的鉴定。BK5-T attP和宿主附着位点B（attB）区域的核心序列在许多乳酸乳球菌噬菌体和乳酸乳球菌菌株中是保守的。
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