Furutani Y, Notake M, Yamayoshi M, Yamagishi J, Nomura H, Ohue M, Furuta R, Fukui T, Yamada M, Nakamura S
Nucleic Acids Res. 1985 Aug 26;13(16):5869-82. doi: 10.1093/nar/13.16.5869.
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.
通过mRNA杂交-翻译试验，从用诱导的兔肺泡巨噬细胞中部分纯化的聚腺苷酸加尾RNA构建的cDNA文库中，克隆出了与兔白细胞介素-1前体（preIL-1）的mRNA互补的DNA序列。以该cDNA为探针，从用诱导的HL-60细胞（一种人单核细胞样细胞系）的聚腺苷酸加尾RNA制备的cDNA文库中分离出人白细胞介素-1 cDNA。从cDNA序列推导的人和兔preIL-1的氨基酸序列揭示了它们的一级结构，分别由271和267个氨基酸残基组成。人和兔之间的氨基酸序列保守性为64%。氨基酸残基数量的差异是由于人preIL-1的羧基末端延伸了4个氨基酸残基。克隆的人cDNA在大肠杆菌中的表达产生了具有生物活性的白细胞介素-1。
