Mori S, Goto F, Goto K, Ohkawara S, Maeda S, Shimada K, Yoshinaga M
Department of Pathology, Kumamoto University Medical School, Japan.
Biochem Biophys Res Commun. 1988 Feb 15;150(3):1237-43. doi: 10.1016/0006-291x(88)90761-9.
Complementary DNA for a rabbit PMN-derived lymphocyte proliferation potentiating factor (PMN factor) was cloned from a cDNA library constructed from poly(A)+RNA of early inflammatory exudate PMN. Oligodeoxyribonucleotides as probes for cloning were synthesized according to the previously reported amino acid sequence of the purified PMN factor. This cDNA encodes a 268-residue protein which is homologous to the established structures of human IL 1 beta (74%0 or murine IL 1 beta (71%). The NH2-terminal amino acid sequence of the endogenously produced PMN factor indicates that the mature molecule is made up of carboxy terminal 152 amino acids of the precursor molecule. Taking into consideration the previous studies on biological activities, this cloned PMN factor is therefore considered to be rabbit IL 1 beta.
从早期炎性渗出物中性粒细胞的聚腺苷酸加尾RNA构建的cDNA文库中，克隆出了兔中性粒细胞衍生的淋巴细胞增殖增强因子（PMN因子）的互补DNA。用于克隆的寡脱氧核糖核苷酸探针是根据先前报道的纯化PMN因子的氨基酸序列合成的。该cDNA编码一种由268个氨基酸残基组成的蛋白质，它与已确定结构的人白细胞介素1β（74%）或小鼠白细胞介素1β（71%）同源。内源性产生的PMN因子的氨基末端氨基酸序列表明，成熟分子由前体分子的羧基末端152个氨基酸组成。考虑到先前关于生物活性的研究，因此该克隆的PMN因子被认为是兔白细胞介素1β。
