Department of Obstetrics and Gynecology, University of New Mexico School of Medicine, MSC 10 5580, 1 University of New Mexico, Albuquerque, NM, 87131, USA.
Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
BMC Pregnancy Childbirth. 2018 Jun 26;18(1):261. doi: 10.1186/s12884-018-1899-6.
Investigators have hypothesized that omega-3 fatty acid supplementation may modulate the immune response. However, available evidence is conflicting. We performed this study to investigate the effect of prenatal eicosapentaenoic acid (EPA)- and docosahexaenoic acid (DHA)-rich fish oil supplementation on maternal and fetal cytokine production.
This study is a secondary analysis of a randomized controlled trial designed to assess whether prenatal EPA- or DHA-rich fish oil supplementation would prevent perinatal depressive symptoms among women at risk. Enrolled participants received EPA-rich fish oil (1060 mg EPA plus 274 mg DHA), DHA-rich fish oil (900 mg DHA plus 180 mg EPA) or soy oil placebo. Maternal venous blood was collected at enrollment (12-20 weeks gestation) and after supplementation (34-36 weeks gestation). Umbilical cord blood was collected at delivery. We analyzed stored plasma specimens for 16 human cytokines using multiplex immunoassays. Maternal and cord blood cytokine levels were compared among the treatment groups. Associations of serum DHA and EPA with maternal and cord blood cytokines were explored via regression analysis.
We enrolled 126 women, of whom 118 completed the trial. Prenatal supplementation with EPA-rich fish oil significantly lowered maternal IL6, IL15, and TNFα concentrations. However, supplementation with DHA-rich fish oil had no significant effect on maternal cytokine profiles. Maternal serum DHA fraction was significantly associated with IL1α, and maternal serum DHA and EPA fractions were significantly associated with IL 10 concentrations after supplementation. Compared with placebo, supplementation with EPA- or DHA-rich fish oils had no significant effect on cord blood cytokine concentrations.
Prenatal supplementation with EPA-rich fish oil significantly reduced levels of several inflammatory cytokines in maternal plasma, while prenatal DHA-rich fish oil had no significant effect on cytokine concentrations. Supplementation with EPA- and DHA- rich fish oil had no significant effect on umbilical cord blood cytokine concentrations.
Clinical Trial Registration: registration number NCT00711971 7/7/2008.
研究人员假设，ω-3 脂肪酸补充剂可能会调节免疫反应。然而，现有的证据存在矛盾。我们进行这项研究是为了调查产前二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）丰富的鱼油补充对产妇和胎儿细胞因子产生的影响。
这是一项随机对照试验的二次分析，旨在评估产前 EPA 或 DHA 丰富的鱼油补充是否会预防有风险的女性围产期抑郁症状。入组参与者接受 EPA 丰富的鱼油（1060mg EPA 加 274mg DHA）、DHA 丰富的鱼油（900mg DHA 加 180mg EPA）或大豆油安慰剂。在入组时（妊娠 12-20 周）和补充后（妊娠 34-36 周）采集母体静脉血。使用多重免疫测定法分析储存的血浆标本中的 16 种人细胞因子。比较治疗组之间母血和脐血细胞因子水平。通过回归分析探讨血清 DHA 和 EPA 与母血和脐血细胞因子的相关性。
我们招募了 126 名女性，其中 118 名完成了试验。产前补充 EPA 丰富的鱼油显著降低了产妇 IL6、IL15 和 TNFα 浓度。然而，DHA 丰富的鱼油补充对产妇细胞因子谱没有显著影响。母体血清 DHA 分数与 IL1α 显著相关，母体血清 DHA 和 EPA 分数与补充后 IL10 浓度显著相关。与安慰剂相比，EPA 或 DHA 丰富的鱼油补充对脐血细胞因子浓度没有显著影响。
产前补充 EPA 丰富的鱼油显著降低了母体血浆中几种炎症细胞因子的水平，而 DHA 丰富的鱼油对细胞因子浓度没有显著影响。EPA 和 DHA 丰富的鱼油补充对脐血细胞因子浓度没有显著影响。
临床试验注册：注册号 NCT00711971，注册日期为 2008 年 7 月 7 日。
