School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai 200240, China.
Division of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26506, USA.
Oxid Med Cell Longev. 2019 Jun 2;2019:6765803. doi: 10.1155/2019/6765803. eCollection 2019.
Pregnancy is an oxidative stress and immune challenge for the mother. Fish oil is rich in EPA and DHA, which can partly inhibit aspects of inflammation and restore antioxidant capacity. In the present study, we investigated the effect of maternal diet with fish oil during the late gestation period on oxidative stress and inflammatory response in sows and their progenies. Twelve second-parity sows were allocated equally into two groups. Sows were fed either the soybean oil diet (SD) or soybean oil+fish oil diet (FD) during the gestation period. The plasma of sows, cord blood, and new-born piglets were collected. Full-term placentas and livers of new-born piglets were also sampled. The activities of glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in the plasma of sows on farrowing day were higher, and the concentrations of interleukin-6 (IL-6) and prostaglandin-endoperoxide synthase 2 (PGE2) in the plasma of sows on farrowing day and interleukin-1 (IL-1) in the plasma of cord blood were lower in the FD group than those in the SD group ( < 0.05). The FD downregulated the expression of SOD, IL-1, IL-6, and transforming growth factor--activated kinase 1 binding protein 1 (TAB1) mRNA but upregulated the expression of lipoxygenase enzyme 5 (ALOX5) and interleukin-10 (IL-10) mRNA in placentas ( < 0.05). The FD downregulated the protein expression level of p-JNK/JNK in placentas ( < 0.05). In the livers of new-born piglets, the FD upregulated the expression of ALOX5 ( < 0.05) and G-protein-coupled receptor 120 (GPR120) ( < 0.05) mRNA. Our results suggest that the maternal diet with fish oil might alleviate oxidative stress in sows on farrowing day and modulate inflammatory response in full-term placentas by inhibiting the JNK signal pathway. Moreover, the maternal diet with fish oil might partly regulate the neonatal immune response of their progenies.
妊娠对母亲来说是一种氧化应激和免疫挑战。鱼油富含 EPA 和 DHA，可部分抑制炎症的某些方面并恢复抗氧化能力。在本研究中，我们研究了在妊娠后期用鱼油喂养的母鼠对母猪及其后代的氧化应激和炎症反应的影响。将 12 头二胎母猪平均分配到两组。母猪在妊娠期间分别喂食大豆油饮食（SD）或大豆油+鱼油饮食（FD）。收集母猪、脐带血和新生仔猪的血浆。还采集了足月胎盘和新生仔猪的肝脏。在分娩当天，FD 组母猪血浆中谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）的活性较高，而在分娩当天和脐带血中母猪血浆中的白细胞介素-6（IL-6）和前列腺素内过氧化物合酶 2（PGE2）以及白细胞介素-1（IL-1）的浓度较低（<0.05）。FD 下调了 SOD、IL-1、IL-6 和转化生长因子激活激酶 1 结合蛋白 1（TAB1）mRNA 的表达，但上调了胎盘白细胞介素-10（IL-10）mRNA 的表达（<0.05）。FD 下调了胎盘 p-JNK/JNK 的蛋白表达水平（<0.05）。在新生仔猪的肝脏中，FD 上调了 ALOX5（<0.05）和 G 蛋白偶联受体 120（GPR120）（<0.05）mRNA 的表达。我们的结果表明，用鱼油喂养的母鼠可能通过抑制 JNK 信号通路减轻分娩当天母猪的氧化应激，并调节足月胎盘的炎症反应。此外，用鱼油喂养的母鼠可能部分调节其后代的新生儿免疫反应。
