Kelly R B
Science. 1985 Oct 4;230(4721):25-32. doi: 10.1126/science.2994224.
Protein secretion from cells can take several forms. Secretion is constitutive if proteins are secreted as fast as they are synthesized. In regulated secretion newly synthesized proteins destined for secretion are stored at high concentration in secretory vesicles until the cell receives an appropriate stimulus. When both constitutive and regulated protein secretion can take place in the same cell a mechanism must exist for sorting the correct secretory protein into the correct secretory vesicle. The secretory vesicle must then be delivered to the appropriate region of plasma membrane. Transfection of DNA encoding foreign secretory proteins into regulated secretory cells has provided insight into the specificity of sorting into secretory vesicles.
细胞的蛋白质分泌可以有几种形式。如果蛋白质一合成便被分泌出去，那么这种分泌就是组成型的。在调节型分泌中，新合成的、 destined for secretion（此处可能有误，应为“ destined for secretion”，即“ destined to be secreted”，意为“注定要被分泌的”）的蛋白质会以高浓度储存在分泌小泡中，直到细胞接收到适当的刺激。当组成型和调节型蛋白质分泌能在同一细胞中发生时，必然存在一种机制，用于将正确的分泌蛋白分选到正确的分泌小泡中。然后，分泌小泡必须被运送到质膜的适当区域。将编码外源分泌蛋白的DNA转染到调节型分泌细胞中，有助于深入了解分选到分泌小泡中的特异性。
