Lian Xiaojie, Liu Shichao, Liu Liming, Xu Rui, Du Miaomiao, Wang Song, Zhu Hesun, Lu Qiang, Zhang Quanyou, Wu Yali, Huang Di, Wei Yan
Department of Biomedical Engineering, Research Center for Nano-Biomaterials and Regenerative Medicine, College of Mechanics, Taiyuan University of Technology, Taiyuan 030024, China.
Shanxi Key Laboratory of Material Strength and Structural Impact, Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Regen Biomater. 2018 Jun;5(3):151-157. doi: 10.1093/rb/rby004. Epub 2018 Mar 15.
With the development of biomaterials, more attention is paid to the adhesion characteristics between cells and materials. It is necessary to study the adhesive force with a suitable method. Silk fibroin (SF) is widely investigated in biomedical application due to its novel biocompatibility and mechanical properties. In this article, the micropipette aspiration method and measurement pattern of uniform cells in round shape (UCR) was used to study the initial adhesive force of three types of cells on pure silk fibroin films (SFFs). We also compared the adhesive forces of modified SFFs with that of pure SFFs. The results of adhesive force in the initial adhesive stage were in concordance with the results of MTT assay and microscope observation, which were confirmed by the above three cell lines and four kinds of SFFs. The results indicated UCR was an efficient and quantitative measurement pattern in initial adhesion stage. This article also provides a useful method in identifying initial cell-materials interactions.
随着生物材料的发展，细胞与材料之间的粘附特性受到了更多关注。有必要用合适的方法来研究粘附力。丝素蛋白（SF）因其新颖的生物相容性和机械性能而在生物医学应用中得到广泛研究。在本文中，采用微吸管抽吸法和圆形均匀细胞（UCR）测量模式来研究三种细胞在纯丝素蛋白膜（SFFs）上的初始粘附力。我们还比较了改性SFFs与纯SFFs的粘附力。初始粘附阶段的粘附力结果与MTT法和显微镜观察结果一致，这得到了上述三种细胞系和四种SFFs的证实。结果表明，UCR是初始粘附阶段一种高效且定量的测量模式。本文还提供了一种识别细胞与材料初始相互作用的有用方法。
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