单细胞非编码 RNA 在胚胎发育中的作用。

Single-Cell Non-coding RNA in Embryonic Development.

机构信息

Department of Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan Province, China.

出版信息

Adv Exp Med Biol. 2018;1068:19-32. doi: 10.1007/978-981-13-0502-3_3.

Abstract

Non-coding RNAs (ncRNAs) have significant regulatory functions on the regulation of gene expression of various life activities after transcription, even though they do not encode proteins. During the development of embryos, ncRNAs, such as long non-coding RNAs (lncRNAs), microRNAs (miRNAs), circular RNAs (circRNAs), small nucleolar RNAs (snoRNAs), and Piwi-interacting RNAs (piRNAs), have been widely proven as key regulators. The emerging single-cell RNA sequencing technique is powerful for profiling "cell-to-cell" variability at the genomic level. It has been applied to detect the expression of ncRNAs during embryo development. In this chapter, we pay close attention to single-cell ncRNA expression and summarize their roles in embryo development.

摘要

非编码 RNA（ncRNAs）在转录后对各种生命活动的基因表达具有重要的调控功能，尽管它们不编码蛋白质。在胚胎发育过程中，ncRNAs，如长非编码 RNA（lncRNAs）、microRNAs（miRNAs）、环状 RNA（circRNAs）、小核仁 RNA（snoRNAs）和 Piwi 相互作用 RNA（piRNAs），已被广泛证明是关键的调控因子。新兴的单细胞 RNA 测序技术在基因组水平上对“细胞间”变异性的分析非常有效。它已被应用于检测胚胎发育过程中 ncRNAs 的表达。在本章中，我们密切关注单细胞 ncRNA 的表达，并总结它们在胚胎发育中的作用。