Jensen M H
Acta Vet Scand. 1985;26(1):72-80. doi: 10.1186/BF03546565.
Three thousand Danish porcine field sera were tested for the possible presence of neutralizing antibodies for Hog Cholera (HC) and Bovine viral Diarrhea (BVD). The technique used was a microplate neutralization test read by peroxidase-linked antibody assay (NPLA) in the HG examination, and by cytopathogenic effect in the BVD examination. The initial dilution of the test sera was 1:2. BVD-antibodies were demonstrated in 6.4 % of the sera, while all the sera were found to be free from antibodies to HG-virus.
检测了3000份丹麦猪的田间血清,以确定是否存在猪霍乱(HC)中和抗体和牛病毒性腹泻(BVD)中和抗体。采用的技术是在HG检测中通过过氧化物酶联抗体测定(NPLA)读取的微孔板中和试验,以及在BVD检测中通过细胞病变效应进行检测。检测血清的初始稀释度为1:2。在6.4%的血清中检测到BVD抗体,而所有血清均未检测到针对HG病毒的抗体。