分析疑似免疫缺陷儿童的总 IgG 与 IgG 亚类总和的差异——决定因素的指示。

Analysis of differences between total IgG and sum of the IgG subclasses in children with suspected immunodeficiency - indication of determinants.

机构信息

3rd Department and Clinic of Paediatrics, Immunology and Rheumatology of Developmental Age, Wroclaw Medical University, L. Pasteura 1, Wroclaw, 50-367, Poland.

Department of Immunology and Paediatrics, Provincial Hospital J. Gromkowski, Koszarowa 5, Wroclaw, 51-149, Poland.

出版信息

BMC Immunol. 2018 Jun 27;19(1):22. doi: 10.1186/s12865-018-0259-7.

DOI:10.1186/s12865-018-0259-7

PMID:29945547

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6020421/

Abstract

BACKGROUND

Deficits in disorders of humoral immunity associated with a deficit of antibodies are the most common primary immunodeficiency. Total IgG and IgG subclasses measurements are used to diagnose, differentiate and control in patients with primary and secondary immunodeficiencies.

METHODS

The purpose of the study was to analyze the structure patients group according to difference between total IgG and sum of the IgG subclasses and to determine factors affecting the level of this difference. This study was based on data collected from 670 children referred to the Department of Clinical Immunology and Pediatrics in order to diagnose the immune disorders. For all children the level of the total of immunoglobulins IgG and of the IgG subclasses (IgG1, IgG2, IgG3, IgG4) were determined. The group of children was divided into subgroups according to gender, age (under 6 years of age, 6.5-12 years, and 12-18 years), and IgG abnormality (below the normal range, normal and above the normal range). In the patients group, the total IgG values were on average higher than sum of the IgG subclasses.

RESULTS

Statistical analysis shown the all parameters under study (age, gender and IgG abnormality) influence statistically significant on the discrepancy between the sum of the IgG subclasses and total IgG. Assessment of IgG and IgG subclasses levels is based on different methods what causes the discrepancy between the sum of the IgG subclasses and total IgG.

CONCLUSIONS

Standardization in that regard is crucial. In addition, we have shown the reliability of the results obtained. Despite the determination in two different laboratories and on different analyzers, as well as the freezing process does not affect the test results.

摘要

背景

与抗体缺陷相关的体液免疫紊乱缺陷是最常见的原发性免疫缺陷。总 IgG 和 IgG 亚类的测量用于诊断、区分和控制原发性和继发性免疫缺陷患者。

方法

本研究的目的是根据总 IgG 与 IgG 亚类总和之间的差异分析患者群体，并确定影响这种差异的因素。这项研究基于从 670 名因免疫失调而被转介到临床免疫和儿科学系的儿童收集的数据。对所有儿童进行总免疫球蛋白 IgG 和 IgG 亚类（IgG1、IgG2、IgG3、IgG4）水平的测定。根据性别、年龄（<6 岁、6.5-12 岁和 12-18 岁）和 IgG 异常（低于正常值、正常值和高于正常值）将儿童分为亚组。在患者组中，总 IgG 值平均高于 IgG 亚类总和。