不能固氮的棕色固氮菌突变菌株的遗传分析。
Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen.
作者信息
Bishop P E, Brill W J
出版信息
J Bacteriol. 1977 May;130(2):954-6. doi: 10.1128/jb.130.2.954-956.1977.
Abstract
Transformation was used to perform ratio test crosses with mutant strains of Azotobacter vinelandii unable to fix N2. Mutations that simultaneously eliminated both components of nitrogenase (nif-1 and nif-2) were tightly linked. The nif-45 mutation that resulted in the absence of an active molybdenum cofactor was closer to nif-1 and nif-2 than to any of the other nif mutations. Strains that lacked component I carried mutations that were closely linked to each other. Mutations that probably were located in the structural genes for components I and II appeared to be relatively close to each other on the A. vinelandii genome.
摘要
利用转化法对不能固氮的棕色固氮菌突变株进行比率测交。同时消除固氮酶两个组分（nif-1和nif-2）的突变紧密连锁。导致缺乏活性钼辅因子的nif-45突变比其他任何nif突变更靠近nif-1和nif-2。缺乏组分I的菌株携带的突变彼此紧密连锁。可能位于组分I和II结构基因中的突变在棕色固氮菌基因组上似乎彼此相对靠近。
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