猪分解代谢素刺激前列腺素E2分泌，但不影响猪滑膜成纤维细胞内的环磷酸腺苷生成。
Porcine catabolin stimulates prostaglandin E2 secretion but does not affect intracellular cyclic AMP production in pig synovial fibroblasts.
作者信息
Carroll G J
出版信息
Ann Rheum Dis. 1985 Sep;44(9):631-6. doi: 10.1136/ard.44.9.631.
Abstract
Responses in vitro to partially purified porcine leucocyte catabolin were studied in pig synovial fibroblasts.In serum-free cultures catabolin was found to stimulate secretion of prostaglandin E2 (PGE2) in a time and concentration-dependent manner. The initial stimulation of PGE2 secretion occurred only after a latent interval of six hours. In the same cell line catabolin was found to have no effect on the production of cyclic adenosine monophosphate (cAMP) at times ranging from 30 s to 20 min, even at concentrations up to 15 times greater than that required to promote accelerated release of glycosaminoglycans from cultured bovine nasal cartilage. It is therefore concluded that in pig synovial fibroblasts catabolin evokes a delayed secretion of PGE2 but does not alter cyclic AMP production.
摘要
在猪滑膜成纤维细胞中研究了其对部分纯化的猪白细胞分解代谢产物的体外反应。在无血清培养中，发现分解代谢产物以时间和浓度依赖的方式刺激前列腺素E2（PGE2）的分泌。PGE2分泌的初始刺激仅在6小时的潜伏期后才出现。在同一细胞系中，发现分解代谢产物在30秒至20分钟的时间范围内对环磷酸腺苷（cAMP）的产生没有影响，即使浓度高达促进从培养的牛鼻软骨中加速释放糖胺聚糖所需浓度的15倍。因此得出结论，在猪滑膜成纤维细胞中，分解代谢产物引起PGE2的延迟分泌，但不会改变cAMP的产生。
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本文引用的文献
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Biochem Biophys Res Commun. 1980 Oct 16;96(3):1225-31. doi: 10.1016/0006-291x(80)90082-0.
2
Human mononuclear cell factors mediate cartilage matrix degradation through chondrocyte activation.人类单核细胞因子通过软骨细胞激活介导软骨基质降解。
J Clin Invest. 1981 Sep;68(3):571-81. doi: 10.1172/jci110290.
3
Lymphocytes induce resorption of cartilage by producing catabolin.淋巴细胞通过产生分解代谢素诱导软骨吸收。
Biochem J. 1982 Jan 15;202(1):275-8. doi: 10.1042/bj2020275.
4
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Biochem J. 1983 Feb 1;209(2):337-44. doi: 10.1042/bj2090337.
5
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Ann Rheum Dis. 1982 Oct;41(5):463-8. doi: 10.1136/ard.41.5.463.
6
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