Boston University, Materials Science and Engineering Division, Boston, 02446, USA.
University of Gothenburg, Department of Medical Biochemistry and Cell Biology, Göteborg, 41390, Sweden.
Sci Rep. 2018 Jun 26;8(1):9710. doi: 10.1038/s41598-018-27732-3.
We present particle tracking microrheology results on human mucins, isolated from normal surface and gland mucosa and one tumor sample, and examine the motility of Helicobacter pylori in these mucins. At 1.5% concentration human mucin solutions are purely viscous, with viscosity η (gland mucin) > η (surface mucin) > η (tumor mucin). In the presence of motile H. pylori bacteria, particle diffusion is enhanced, with diffusivity D(tumor mucin) > D(gland mucin) > D(surface mucin). The surface and tumor mucin solutions exhibit an elastic response in the presence of bacteria. Taken together these results imply that particle diffusion and active swimming are coupled and impact the rheology of mucin solutions. Both J99 wild type (WT) and its isogenic ΔbabA/ΔsabA mutant swam well in broth or PGM solutions. However, the human mucins affected their motility differently, rendering them immotile in certain instances. The distribution of swimming speeds in human mucin solutions was broader with a large fraction of fast swimmers compared to PGM and broth. The bacteria swam fastest in the tumor mucin solution correlating with it having the lowest viscosity of all mucin solutions. Overall, these results suggest that mucins from different tissue locations and disease status differ in their microrheological properties and their effect on H. pylori motility.
我们展示了人粘蛋白的粒子追踪微流变学结果，这些粘蛋白分别来自正常表面和腺体粘膜以及一个肿瘤样本，并研究了幽门螺杆菌在这些粘蛋白中的运动情况。在 1.5%浓度下，人粘蛋白溶液是纯粘性的，粘度 η（腺体粘蛋白）> η（表面粘蛋白）> η（肿瘤粘蛋白）。在运动的幽门螺杆菌细菌存在下，颗粒扩散增强，扩散系数 D（肿瘤粘蛋白）> D（腺体粘蛋白）> D（表面粘蛋白）。表面和肿瘤粘蛋白溶液在存在细菌时表现出弹性响应。这些结果表明，颗粒扩散和主动游动是耦合的，并影响粘蛋白溶液的流变学。J99 野生型（WT）及其同源的 ΔbabA/ΔsabA 突变体在肉汤或 PGM 溶液中都能很好地游动。然而，人粘蛋白对它们的运动有不同的影响，使它们在某些情况下变得不动。与 PGM 和肉汤相比，在人粘蛋白溶液中，游动速度的分布更广泛，快速游动的细菌比例更大。细菌在肿瘤粘蛋白溶液中游动最快，与所有粘蛋白溶液中最低的粘度相对应。总的来说，这些结果表明，来自不同组织位置和疾病状态的粘蛋白在它们的微观流变学特性及其对幽门螺杆菌运动的影响方面存在差异。
