Department of Biosystems, MeBioS-Biosensors group , KU Leuven-University of Leuven , Willem de Croylaan 42 , B-3001 Leuven , Belgium.
Centre for Medical Biotechnology (ZMB) , University of Duisburg-Essen , Universitätstrasse 2 , 45117 Essen , Germany.
ACS Appl Mater Interfaces. 2018 Jul 18;10(28):23539-23547. doi: 10.1021/acsami.8b04757. Epub 2018 Jul 5.
Many challenges in biosensing originate from the fact that the all-important nanoarchitecture of the biosensor surface, including precise density and orientation of bioreceptors, is not entirely comprehended. Here, we introduced a three-dimensional DNA origami as a bioreceptor carrier to functionalize the fiber optic surface plasmon resonance (FO-SPR) sensor with nanoscale precision. Starting from a 24-helix bundle, two distinct DNA origami structures were designed to position thrombin-specific aptamers with different densities and distances (27 and 113 nm) from the FO-SPR surface. The origami-based biosensors not only proved to be capable of reproducible, label-free thrombin detection but revealed also valuable innovative features: (1) a significantly better performance in the absence of backfilling, known as essential in the biosensing field, suggesting improved bioreceptor orientation and accessibility, and (2) a wider linear range compared to previously reported thrombin biosensors. We envisage that our method will be beneficial for both scientists and clinicians looking for new surface (bio)chemistry and improved diagnostics.
许多生物传感方面的挑战源于这样一个事实，即生物传感器表面至关重要的纳米结构，包括生物受体的精确密度和取向，还没有完全被理解。在这里，我们引入了一种三维 DNA 折纸作为生物受体载体，以纳米级精度对光纤表面等离子体共振（FO-SPR）传感器进行功能化。从 24 螺旋束开始，设计了两种不同的 DNA 折纸结构，以从 FO-SPR 表面以不同的密度和距离（27nm 和 113nm）定位凝血酶特异性适体。基于折纸的生物传感器不仅被证明能够进行可重复的、无标记的凝血酶检测，而且还具有有价值的创新性特征：（1）在没有回填的情况下性能显著提高，这在生物传感领域是必不可少的，表明生物受体的取向和可及性得到了改善；（2）与之前报道的凝血酶生物传感器相比，线性范围更宽。我们设想，我们的方法将有益于寻找新的表面（生物）化学和改进诊断的科学家和临床医生。
