Department of Zoology, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India.
Biol Trace Elem Res. 2019 Feb;187(2):543-552. doi: 10.1007/s12011-018-1407-x. Epub 2018 Jun 9.
This study deals with synthesis of chromium nanoparticles (CrNPs) from potassium dichromate using the aqueous extract of Allium sativum. They were characterized through UV-VIS light, FE-SEM, EDX, XRD, and FT-IR, which revealed uniform, mono-dispersive, and highly stable CrNPs of 31-64-nm size. The Artemia nauplii was enriched with 4.94 mg/L of CrNPs (24-h LC) at different durations (½, 1, 2, and 4 h) and then fed to Macrobrachium rosenbegii post-larvae (PL) for 30 days as live feed. The results showed that ½- and 1-h enriched Artemia nauplii led to significant improvements in nutritional indices including growth and survival, and concentrations of tissue biochemical constituents, such as total protein, amino acid, carbohydrate, and lipid of M. rosenbergii PL (P < 0.05), which suggests that this concentration of CrNPs was non-toxic to M. rosenbergii PL. This was confirmed by the insignificant alterations recorded in activities of SOD and CAT (P > 0.05) in M. rosenbergii PL fed with ½- and 1-h enriched Artemia nauplii as live feed. After that, SOD and CAT activities started to increase. Therefore, this optimized concentration of CrNPs (4.94 mg/L) is recommended for enrichment of Artemia nauplii for ½-1-h duration as a sustainable material in the nursery of M. rosenbergii.
本研究使用大蒜的水提物从重铬酸钾合成了铬纳米粒子（CrNPs）。通过 UV-VIS 光、FE-SEM、EDX、XRD 和 FT-IR 对它们进行了表征，结果表明 CrNPs 具有均匀、单分散性和高度稳定性，粒径为 31-64nm。用不同时间（½、1、2 和 4 小时）富集了 4.94mg/L CrNPs（24 小时 LC）的卤虫无节幼体，然后作为活饲料喂养罗氏沼虾幼体（PL）30 天。结果表明，½ 小时和 1 小时富集的卤虫无节幼体显著提高了罗氏沼虾幼体的营养指标，包括生长和存活率，以及组织生化成分的浓度，如总蛋白、氨基酸、碳水化合物和脂质（P<0.05），这表明该浓度的 CrNPs 对罗氏沼虾幼体无毒。这通过未记录到 SOD 和 CAT 活性在食用½ 小时和 1 小时富集的卤虫无节幼体作为活饲料的罗氏沼虾幼体中发生明显变化（P>0.05）得到证实。之后，SOD 和 CAT 活性开始增加。因此，建议将该优化浓度的 CrNPs（4.94mg/L）用于富集卤虫无节幼体，时间为 ½-1 小时，作为罗氏沼虾幼体育苗的可持续材料。
