Departamento de Ciencias Biológicas Animales, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santa Rosa 11735, La Pintana, Santiago, Chile.
Appl Biochem Biotechnol. 2019 Jan;187(1):101-115. doi: 10.1007/s12010-018-2805-4. Epub 2018 Jun 13.
Avian eggshell membranes are good candidates as a matrix for immobilization procedures. Chicken eggshell, a waste material available from the poultry industry as a byproduct, is a very safe and cheap raw material. While pieces of eggshell membrane, or even particles from whole eggshell, have been previously used for these purposes, we report here the use of eggshell membrane powder for E. coli β-galactosidase immobilization with glutaraldehyde as cross-linker. A kinetic characterization is provided for eggshell membrane powder-bound enzyme compared to free enzyme. Results show a remarkable similarity between bound and free enzyme and also that the immobilized enzyme is stable and can be reused several times. Moreover, bound enzyme is able to produce glucose from skim milk serum.
禽类蛋壳膜是固定化程序的理想基质。鸡蛋壳是家禽业的一种副产物，来源广泛且非常安全、廉价。虽然此前曾使用蛋壳膜片甚至整个蛋壳的颗粒来达到这些目的，但我们在此报告使用蛋壳膜粉末作为载体，戊二醛作为交联剂固定化大肠杆菌β-半乳糖苷酶。与游离酶相比，对蛋壳膜粉末结合酶进行了动力学特征分析。结果表明，结合酶和游离酶之间具有显著的相似性，并且固定化酶稳定，可重复使用多次。此外，结合酶能够从脱脂乳血清中产生葡萄糖。
