Hall M, Mickey D D, Wenger A S, Silverman L M
Clin Chem. 1985 Oct;31(10):1689-91.
Data are presented demonstrating that adenylate kinase (AK; EC 2.7.4.3) is an oncodevelopmental enzyme in the prostate of Copenhagen rats. We selected the Dunning tumor (dorsal rat prostate) as a model system because it most nearly approximates the human pathology. Four sublines of the tumor (R3327-H, R3327-AT, MAT Lu, and MAT LyLu) were studied. The tumor sublines were maintained as solid tumors in syngeneic rats and as monolayers in tissue culture. AK activity appeared in conjunction with malignant transformation of the dorsal prostate. We also determined the normal developmental enzyme pattern: AK was present in prostates of newborns, but was undetectable in prostates of adults. However, AK increased after castration. Therefore, we propose AK as a potential oncofetal tumor marker in prostatic cancer.
数据表明，腺苷酸激酶（AK；EC 2.7.4.3）是哥本哈根大鼠前列腺中的一种肿瘤发生发展相关酶。我们选择邓宁肿瘤（大鼠背侧前列腺）作为模型系统，因为它最接近人类病理学情况。对该肿瘤的四个亚系（R3327 - H、R3327 - AT、MAT Lu和MAT LyLu）进行了研究。肿瘤亚系在同基因大鼠中作为实体瘤维持，并在组织培养中作为单层细胞维持。AK活性与背侧前列腺的恶性转化同时出现。我们还确定了正常发育过程中的酶模式：AK存在于新生儿前列腺中，但在成年前列腺中无法检测到。然而，去势后AK增加。因此，我们提出AK作为前列腺癌潜在的癌胚肿瘤标志物。
