Department of Thoracic Surgery, Affiliated Hospital of Jining Medical University, Jining, Shandong, China.
Eur Rev Med Pharmacol Sci. 2018 Jun;22(12):3789-3794. doi: 10.26355/eurrev_201806_15261.
Up-regulation of miR-765 in esophageal squamous cell carcinoma (ESCC) has been reported in the previous study. The aim of the present study was to measure the levels of miR-765 expression in ESCC and evaluate its clinical significance in ESCC patients.
Quantitative Real-time PCR assays were performed to analyze the expression of miR-765 in human ESCC tissues and adjacent esophageal tissues. The relationships between miR-765 expression levels and the clinical factors were investigated by x2-test. Kaplan-Meier analysis was performed to evaluate the overall survival (OS) and disease-free survival (DFS) of ESCC patients with a different expression level of miR-765. The Cox proportional hazards regression model was used to assess the independent prognostic factors.
The expression level of miR-765 in ESCC tissues was significantly higher than that in their corresponding normal tissues (p < 0.01). High miR-765 expression was significantly correlated with tumor stage (p = 0.001), lymph nodes metastasis (p = 0.005), clinical stage (p = 0.007). In addition, Kaplan-Meier analysis showed that patients with higher miR-765 expression had a significantly poorer OS (p = 0.0010) and DFS (p< 0.0001) than those with lower miR-765 expression. Multivariate analyses revealed that miR-765 expression served as an independent predictor for both OS (p = 0.001) and DFS (p = 0.001).
Our findings provided the first evidence that miR-765 may serve as an indicator for prognosis of ESCC.
先前的研究报道食管鳞癌（ESCC）中 miR-765 的表达上调。本研究旨在测量 ESCC 中 miR-765 的表达水平，并评估其在 ESCC 患者中的临床意义。
采用定量实时 PCR 检测分析人 ESCC 组织和相邻食管组织中 miR-765 的表达。x2 检验分析 miR-765 表达水平与临床因素的关系。Kaplan-Meier 分析评估 miR-765 不同表达水平的 ESCC 患者的总生存期（OS）和无病生存期（DFS）。Cox 比例风险回归模型评估独立的预后因素。
ESCC 组织中 miR-765 的表达水平明显高于相应的正常组织（p < 0.01）。高 miR-765 表达与肿瘤分期（p = 0.001）、淋巴结转移（p = 0.005）、临床分期（p = 0.007）显著相关。此外，Kaplan-Meier 分析显示，miR-765 高表达患者的 OS（p = 0.0010）和 DFS（p< 0.0001）明显低于 miR-765 低表达患者。多变量分析显示，miR-765 表达是 OS（p = 0.001）和 DFS（p = 0.001）的独立预测因素。
我们的研究结果首次提供证据表明，miR-765 可能是 ESCC 预后的一个指标。
