Zhang Bao-Jun, Gong Hong-Yan, Zheng Fang, Liu De-Jun, Liu Hai-Xia
Department of Anesthesiology, Central Hospital of Xinxiang City Xinxiang 453000, China.
Department of Anesthesiology, The First Affiliated Hospital of Xinxiang Medical University Weihui 453100, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6213-8. eCollection 2014.
MicroRNAs (miRNAs) are noncoding RNAs that regulate multiple cellular processes during cancer progression. MiR-335 has recently been identified to be involved in tumorigenesis of several cancers such as ovarian cancer and gastric cancer. However, the regulation of miR-335 in esophageal squamous cell carcinoma (ESCC) has not been reported yet.
Expression of miR-335 in tumor and their normal matched tissues was determined by quantitative real-time PCR in 67 ESCC patients and its association with overall survival of patients was analyzed by statistical analysis.
The expression level of miR-335 was reduced in malignant tissue samples in comparison to normal matched tissue (P < 0.05). It was also proved that miR-335 expression was associated with ESCC histological grade, lymph node metastasis, tumor stage and clinical stage (P < 0.05). In addition, the Kaplan-Meier survival curves revealed that low miR-335 expression was associated with poor prognosis in ESCC patients. Multivariate analysis showed that miR-335 expression was an independent prognostic marker of overall survival of ESCC patients.
The study proves for the first time that miR-335 is down regulated in a majority of ESCC patients. Our results indicate that miR-335 expression is an independent prognostic factor for patients with esophageal cancer, which might be a potential valuable biomarker for ESCC.
微小RNA（miRNA）是非编码RNA，在癌症进展过程中调节多种细胞过程。最近已确定miR-335参与卵巢癌和胃癌等多种癌症的肿瘤发生。然而，miR-335在食管鳞状细胞癌（ESCC）中的调节作用尚未见报道。
通过定量实时PCR测定67例ESCC患者肿瘤及其正常配对组织中miR-335的表达，并通过统计分析分析其与患者总生存的关系。
与正常配对组织相比，恶性组织样本中miR-335的表达水平降低（P < 0.05）。还证明miR-335表达与ESCC组织学分级、淋巴结转移、肿瘤分期和临床分期相关（P < 0.05）。此外，Kaplan-Meier生存曲线显示，miR-335低表达与ESCC患者预后不良相关。多因素分析表明，miR-335表达是ESCC患者总生存的独立预后标志物。
该研究首次证明大多数ESCC患者中miR-335表达下调。我们的结果表明，miR-335表达是食管癌患者的独立预后因素，可能是ESCC潜在的有价值生物标志物。
