Department of Anorectal Surgery, Weifang People's Hospital, No.151, Guangwen Street, Weifang, 261000, Shandong, China.
World J Surg Oncol. 2022 Mar 8;20(1):73. doi: 10.1186/s12957-022-02529-x.
The incidence and mortality of colon cancer is increasing recently. It is necessary to identify effective biomarkers for the progression and prognosis of colon cancer. To assess the potential of lncRNA USP30-AS1 (USP30-AS1) in serving as the biomarker of colon cancer and unearth the underlying mechanism.
There were 123 colon cancer patients enrolled. The expression of USP30-AS1 was evaluated with PCR in tissue and cell samples. The clinical significance of USP30-AS1 was assessed with a series of statistical methods, while the CCK8 and Transwell assay were conducted to estimate its biological effect on the colon cancer cellular processes. In mechanism, the interaction of USP30-AS1 with miR-765 was evaluated with the dual-luciferase reporter assay.
In colon cancer tissues, the USP30-AS1 downregulation and the miR-765 upregulation were observed, and there was a negative correlation between the USP30-AS1 expression level and the miR-765 expression level. The downregulation of USP30-AS1 related to the malignant progression and served as an adverse prognostic indicator of colon cancer. The overexpression of USP30-AS1 dramatically suppressed colon cancer cellular processes, which was alleviated by miR-765.
USP30-AS1 predicts the malignancy and prognosis of colon cancer patients. USP30-AS1 suppressed the progression of colon cancer through modulating miR-765.
结肠癌的发病率和死亡率最近呈上升趋势。有必要确定结肠癌进展和预后的有效生物标志物。评估长链非编码 RNA USP30-AS1（USP30-AS1）作为结肠癌标志物的潜力，并揭示其潜在机制。
共纳入 123 例结肠癌患者。采用 PCR 法检测组织和细胞样本中 USP30-AS1 的表达。采用一系列统计学方法评估 USP30-AS1 的临床意义，同时采用 CCK8 和 Transwell 试验评估其对结肠癌细胞过程的生物学效应。在机制方面，通过双荧光素酶报告基因试验评估 USP30-AS1 与 miR-765 的相互作用。
在结肠癌组织中观察到 USP30-AS1 下调和 miR-765 上调，USP30-AS1 表达水平与 miR-765 表达水平呈负相关。USP30-AS1 的下调与恶性进展有关，是结肠癌不良预后的指标。USP30-AS1 的过表达显著抑制结肠癌细胞过程，而 miR-765 可减轻其抑制作用。
USP30-AS1 可预测结肠癌患者的恶性程度和预后。USP30-AS1 通过调节 miR-765 抑制结肠癌的进展。
