聚合物基质内纳米颗粒标记移植群体的无创成像用于神经细胞治疗。
Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy.
机构信息
Institute for Science & Technology in Medicine, Keele University, Keele, ST5 5BG, UK.
Institute of Translational Medicine, University of Liverpool, Liverpool, L69 3BX, UK.
出版信息
Nanomedicine (Lond). 2018 Jun;13(11):1333-1348. doi: 10.2217/nnm-2017-0347.
AIM
To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI.
MATERIALS & METHODS: Astrocytes were exogenously labeled with MRI-compatible iron-oxide MNPs prior to intra-construct incorporation within a 3D collagen hydrogel.
RESULTS
A connective, complex cellular network was clearly observable within the 3D constructs, with high cellular viability. MNP accumulation in astrocytes provided a hypointense MRI signal at 24 h & 14 days.
CONCLUSION
Our findings support the concept of developing a 3D construct possessing the dual advantages of (i) support of long-term cell survival of neural populations with (ii) the potential for noninvasive MRI-tracking of intra-construct cells for neuroregenerative applications.
目的
开发一种用于封装移植细胞的 3D 神经细胞构建体；开发适合 MRI 细胞跟踪的、种有磁性纳米颗粒 (MNP) 标记细胞的水凝胶。
材料与方法
在 3D 胶原水凝胶中加入外源性标记有 MRI 兼容氧化铁 MNP 的星形胶质细胞。
结果
在 3D 构建体中可以清楚地观察到连接的、复杂的细胞网络，细胞活力很高。星形胶质细胞内 MNP 的积累在 24 小时和 14 天时提供了低信号强度的 MRI 信号。
结论
我们的研究结果支持开发一种 3D 构建体的概念，该构建体具有双重优势：（i）支持神经细胞群体的长期存活，（ii）具有对神经再生应用中构建体内细胞进行非侵入性 MRI 跟踪的潜力。
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